Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation

ABSTRACT: Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Skeletal Muscle, Cell Culture, Hela Cell

SUBMITTER: Tanveer Batth  

LAB HEAD: Jesper Velgaard Olsen

PROVIDER: PXD011677 | Pride | 2019-03-05

REPOSITORIES: Pride