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Revealing mechanisms of mating plug function under sexual selection C4PR_LIV

ABSTRACT: A novel proteomic approach reveals dual functions for rodent mating plugs in sperm competition.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Myodes Glareolus (bank Vole) (clethrionomys Glareolus)

TISSUE(S): Testis, Sperm

SUBMITTER: Catarina Franco

LAB HEAD: Professor Rob Beynon

PROVIDER: PXD011694 | Pride | 2021-09-08

REPOSITORIES: Pride

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			Action	DRS
	12030_1.msf	Msf
	12030_1.raw	Raw
	12030_4.msf	Msf
	12030_4.raw	Raw
	12034_1.msf	Msf

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Publications

Revealing mechanisms of mating plug function under sexual selection.

Stockley Paula P Franco Catarina C Claydon Amy J AJ Davidson Amanda A Hammond Dean E DE Brownridge Philip J PJ Hurst Jane L JL Beynon Robert J RJ

Proceedings of the National Academy of Sciences of the United States of America 20201019 44

Mating plugs are produced by many sexually reproducing animals and are hypothesized to promote male fertilization success under promiscuous mating. However, tests of this hypothesis have been constrained by an inability to discriminate ejaculates of different males in direct competition. Here, we use stable isotope labeling in vivo and proteomics to achieve this in a promiscuous rodent, <i>Myodes glareolus</i> We show that, although the first male's plug is usually dislodged, it can be retained ...[more]

PMID: 33077605

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Revealing mechanisms of mating plug function under sexual selection.

Project description:Mating plugs are produced by many sexually reproducing animals and are hypothesized to promote male fertilization success under promiscuous mating. However, tests of this hypothesis have been constrained by an inability to discriminate ejaculates of different males in direct competition. Here, we use stable isotope labeling in vivo and proteomics to achieve this in a promiscuous rodent, Myodes glareolus We show that, although the first male's plug is usually dislodged, it can be retained throughout the second male's copulation. Retained plugs did not completely block rival sperm but did significantly limit their numbers. Differences in the number of each male's sperm progressing through the female reproductive tract were also explained by natural variation in the size of mating plugs and reproductive accessory glands from which major plug proteins originate. Relative sperm numbers in turn predicted the relative fertilization success of rival males. Our application of stable isotopes to label ejaculates resolves a longstanding debate by revealing how rodent mating plugs promote fertilization success under competitive conditions. This approach opens new opportunities to reveal cryptic mechanisms of postcopulatory sexual selection among diverse animal taxa.

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