Dataset Information

Profiling Protein Expression Levels and Genetically Variant Peptides in Human Hair From Different Body Sites

ABSTRACT: Proteins of the human hair shaft contain a wealth of information about the coding regions of the person’s genome from whom the hair is derived. Commonly found at crime scenes, hair shafts may thus provide useful forensic evidence if the information they contain can be exploited. Present experiments show that hair shafts from four different anatomic sites are similarly useful in distinguishing individuals by protein profiling. However, the results demonstrated that protein profiles were dependent on anatomic site, indicating that a proper comparison requires matching the sites of origin. The differences in profile offer the prospect of determining the site of origin of hair by comparison with profiles of shafts from other anatomic sites. By contrast, the genetically variant peptides detected in the protein digests, that map to non-synonymous single nucleotide polymorphisms in subject DNA, were detectable regardless of the anatomic origin of the hair shafts. The resulting profiles of genetically variant peptides were more dependent on a subject’s genotype than on the anatomic origin of the hair shaft. Individual identification therefore can be based on peptide profiles regardless of body location. This study demonstrates the utility of proteomic analysis for increasing the forensic value of hair shaft evidence.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hair Shaft

SUBMITTER: Glendon Parker

LAB HEAD: Glendon J. Parker

PROVIDER: PXD011732 | Pride | 2019-04-04

REPOSITORIES: Pride

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Comparison of protein expression levels and proteomically-inferred genotypes using human hair from different body sites.

Milan Jennifer A JA Wu Pei-Wen PW Salemi Michelle R MR Durbin-Johnson Blythe P BP Rocke David M DM Phinney Brett S BS Rice Robert H RH Parker Glendon J GJ

Forensic science international. Genetics 20190311

The microanatomy of human hair differs as a function of the site of origin on the body. This was a major consideration when anatomical features of hair were used as a means of comparison and human identification. Recent advances have demonstrated that proteomics of the hair shaft can be used to develop profiles of protein abundance and genetically variant peptides, the latter in turn being used to infer genotypes of SNP alleles. Because the profile of proteins would be expected to change as hair ...[more]

PMID: 30939338

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