Dataset Information

Temporal proteome analyses of the alternative rubber crop Taraxacum koksaghyz

ABSTRACT: Taraxacum koksaghyz is currently one of the most investigated plants as a potential source of natural rubber. This poly(cis-1,4-isoprene) polymer and further valuable secondary metabolites are produced in laticifer cells, which exist as a network of elongated tubules throughout the plant and have a milky cytoplasm known as latex. However, a detailed analysis of the overall laticifer system and a deeper knowledge about the interaction with the surrounding tissues has not been performed. Therefore, we used a bacterial ribonuclease for the development of transgenic dandelion plants with omitted latex production. Comprehensive histological, biochemical and proteomic analyses served to characterize these plants. Thereby, we were able to generate a molecular map based on the protein expression pattern of laticifers and whole Taraxacum koksaghyz roots at different stages of plant development. This enables us to understand the interplay and tissue specificity of different biosynthesis routes and narrow down biological functions of latex.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Taraxacum

TISSUE(S): Root

SUBMITTER: Iris Finkemeier

LAB HEAD: Prof. Dr. Iris Finkemeier

PROVIDER: PXD011773 | Pride | 2020-05-28

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	170606_C17_030_QHF_Standard_Rev02_17_032_PAP73_1.raw	Raw
	170606_C17_030_QHF_Standard_Rev02_17_032_PAP73_2.raw	Raw
	170606_C17_030_QHF_Standard_Rev02_17_032_PAP73_3.raw	Raw
	170606_C17_030_QHF_Standard_Rev02_17_032_PAP74_1.raw	Raw
	170606_C17_030_QHF_Standard_Rev02_17_032_PAP74_2.raw	Raw

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Publications

Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology.

Benninghaus Vincent Alexander VA van Deenen Nicole N Müller Boje B Roelfs Kai-Uwe KU Lassowskat Ines I Finkemeier Iris I Prüfer Dirk D Schulze Gronover Christian C

Journal of experimental botany 20200201 4

Taraxacum koksaghyz has been identified as one of the most promising alternative rubber crops. Its high-quality rubber is produced in the latex of laticifers, a specialized cell type that is organized in a network of elongated tubules throughout the entire plant body. In order to gain insights into the physiological role(s) of latex and hence laticifer biology, we examine the effects of barnase-induced latex RNA degradation on the metabolite and protein compositions in the roots. We established ...[more]

PMID: 31740929

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