Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fibroblast secretome proteomic analysis

ABSTRACT: LV fibroblasts were isolated from control (day 0), and infarcted regions at day 1, day 3, and day 7 after myocardial infarction (MI). Cells were isolated in DMEM in 10% fetal bovine serum and were seeded into 6-well plates and were allowed to grow to 80% confluence in DMEM supplemented with 10% fetal bovine serum (FBS). Cells were serum starved (DMEM + 0.1% FBS) for 18 h and then media was changed and secretome was collected after 24 h. The sample sizes are n=3 biological replicates for each time point.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Heart, Fibroblast

SUBMITTER: Elizabeth Flynn

LAB HEAD: Merry Lindsey

PROVIDER: PXD011778 | Pride | 2019-10-25

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	151051510815082D33.msf	Msf
	151051510815082D33.raw	Raw
	151231524315244D72.msf	Msf
	151231524315244D72.raw	Raw
	15159-15168D012.msf	Msf

Items per page:

1 - 5 of 26

Publications

Fibroblast polarization over the myocardial infarction time continuum shifts roles from inflammation to angiogenesis.

Mouton Alan J AJ Ma Yonggang Y Rivera Gonzalez Osvaldo J OJ Daseke Michael J MJ Flynn Elizabeth R ER Freeman Tom C TC Garrett Michael R MR DeLeon-Pennell Kristine Y KY Lindsey Merry L ML

Basic research in cardiology 20190111 2

Cardiac fibroblasts are the major producers of extracellular matrix (ECM) to form infarct scar. We hypothesized that fibroblasts undergo a spectrum of phenotype states over the course of myocardial infarction (MI) from early onset to scar formation. Fibroblasts were isolated from the infarct region of C57BL/6J male mice (3-6 months old, n = 60) at days 0 (no MI control) and 1, 3, or 7 after MI. Whole transcriptome analysis was performed by RNA-sequencing. Of the genes sequenced, 3371 were differ ...[more]

PMID: 30635789

Similar Datasets

Modulation of the cancer cell transcriptome by culture media formulations and cell density

Project description:We investigated how varying the composition of cell culture formulations and growing cancer cells at different densities might affect tumor cells genotype. Specifically, we compared gene expression profiles generated by human MDA-MB-231 human breast cancer cells cultured in different media (MEM, DMEM, or RPMI 1640) containing different concentrations of fetal bovine serum (FBS) or different sera (equine or bovine) that were grown at different cell densities. We have 6 different group (1. MDA-MB-231; MEM-10% FBS-90% confluence (MEM-1 and MEM-2). 2. MDA-MB-231; DMEM-10% FBS-90% confluence (DMEM-1 and DMEM-2). 3. MDA-MB-231; RPMI1640-10% FBS-90% confluence (RPMI-1 and RPMI-2) 4. MDA-MB-231; MEM-10% Horse serum-90% confluence (Horse-1 and Horse-2). 5. MDA-MB-231; MEM-0.1% FBS-90% confluence (FBS0.1-1 and FBS0.1-2). 6. MDA-MB-231; MEM-10% FBS-50% confluence (Con50-1 and Con50-2). Each group has duplicated. We analyzed; 1. Cell density (90% vs 50% confluence). 2. FBS concentration (10% FBS vs 0.1% FBS). 3. Sera (10% FBS vs 10% Horse serum). 4. Media (MEM vs DMEM vs RPMI1640)

2015-06-17 | E-GEOD-61670 | biostudies-arrayexpress

Proteomic profile of macrophage serectome

Project description:Macrophage secretome- Macrophages were isolated from the left ventricle from control (day 0) or from the infarct region at day 1 after myocardial infarction (MI). Cells were isolated in RPMI media with 0.1% fetal bovine serum and incubated for 2 hours and then spun at 800g for 5 min to separate cell debris. The sample sizes are n=4 biological replicates for each time point.

2022-05-19 | PXD011780 | Pride

Endothelin-1 (ET-1) treated lung fibroblasts

Project description:Primary lung fibroblasts were grown to confluence in DMEM 10% FBS, serum starved in DMEM 0.5% FBS for 18 hours. Cells were then treated in the presence or absence of 100 nM ET-1 in the same medium for an additional 4 hours. Keywords: repeat sample

2004-02-28 | GSE981 | GEO

Comparison of gene expression profiles of miR-142-/- primary megakaryocytes and WT primary megakaryocytes

Project description:Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver cells were maintained in Dulbecco modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, 100µg/ml streptomycin, and 50ng/ml recombinant human thrombopoietin (TPO; Peprotech). After 5 days of culture, megakaryocytes were purified using a discontinuous bovine serum albumin gradient (BSA, Sigma–Aldrich; 3%, 1.5%, and 0%). Total RNA was isolated with Tri–Reagent (MRC) following manufacturer’s instructions, and its quality was assessed with ND–1000 Nanodrop (Peqlab) and on a 1.5% agarose gel.

2014-06-11 | GSE52141 | GEO

Random differentiation of mouse embryonic stem cell

Project description:Embryoid Bodies were dissociated and plated onto tissue culture dish in DMEM with fetal bovine serum and antibiotics for 4, 8, 12, 15, 21 day. Keywords: development stage

2005-11-01 | GSE3527 | GEO

Modulation of the cancer cell transcriptome by culture media formulations and cell density

2015-06-17 | GSE61670 | GEO

Transcriptomic study of NR8383 rat macrophages cells in control condition (DMEM culture medium supplemented with 10% of fetal bovine serum)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages: here is the control for Air Liquid Interface (ALI) in DMEM culture medium supplemented with fetal bovine serum (FBS). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156458 | GEO

Concentration and composition of the protein corona as a function of incubation time and serum concentration

Project description:We describe the concentration and composition of the protein corona as a function of incubation time (30 min and 1 day) and the concentration of fetal bovine serum (FBS) used to form the corona (5%, 10%, and 100%) on 200 nm carboxylate-modified magnetic NPs, using semi-automated processes.

2023-03-21 | PXD035811 | Pride

Comparison of gene expression profiles of miR-142-/- primary megakaryocytes and WT primary megakaryocytes

Project description:Whole fetal livers were collected from mouse fetuses at embryonic day 14.5 (E14.5), and single-cell suspensions were prepared by successive passage through 18-, 21 and 23-gauge needles. Fetal liver cells were maintained in Dulbecco modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 100 U/ml penicillin, 100M-BM-5g/ml streptomycin, and 50ng/ml recombinant human thrombopoietin (TPO; Peprotech). After 5 days of culture, megakaryocytes were purified using a discontinuous bovine serum albumin gradient (BSA, SigmaM-bM-^@M-^SAldrich; 3%, 1.5%, and 0%). Total RNA was isolated with TriM-bM-^@M-^SReagent (MRC) following manufacturerM-bM-^@M-^Ys instructions, and its quality was assessed with NDM-bM-^@M-^S1000 Nanodrop (Peqlab) and on a 1.5% agarose gel. Gene expression was measured in primary megakaryocytes cultured from miR-142-/- fetal livers and wild type fetal livers at E14.5. Two independent experiments were performed.

2014-06-11 | E-GEOD-52141 | biostudies-arrayexpress

Comparison of HEK293T cells grown in serum supplemented media to cells serum starved for 3 days

Project description:HEK293T cells grown to confluence in media +10% fetal bovine serume. Media was removed and replaced with serum free media, and cultured for 3 days. RNA was harvested from day0 (serum supplemented), control, and day3 (serum starved) cultures, experiment.

2014-12-13 | GSE64117 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data