Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of HEK293T cells grown in serum supplemented media to cells serum starved for 3 days

ABSTRACT: HEK293T cells grown to confluence in media +10% fetal bovine serume. Media was removed and replaced with serum free media, and cultured for 3 days. RNA was harvested from day0 (serum supplemented), control, and day3 (serum starved) cultures, experiment.

ORGANISM(S): Homo sapiens

PROVIDER: GSE64117 | GEO | 2014/12/13

SECONDARY ACCESSION(S): PRJNA270185

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Time course of serum starved ARPE19 cells

Project description:Cells from the human retina pigment epithilium cell line, ARPE-19, were cultured in media supplemented with 10% fetal bovine serum. Then, the supplemented media was replaced with serum free media. Cells were collected and RNA prepared from the serum free cultures on days 0, 1, 3, 5, and 7. Changes in gene expression were calculated using the day0 sample for reference.

2014-12-13 | GSE64116 | GEO

Genome-wide analysis of serum starved prelamin A-accumulating hMSCs gene expression.

2014-06-23 | GSE52563 | GEO

MicroRNA transcriptional profiling in serum-starved human aortic smooth muscle cells

Project description:We sought to identify microRNAs that were differentially regulated in cultured primary human aortic smooth muscle cells (SMCs) when exposed to growth arrest via serum starvation over two time points - 48 hours and 72 hours, when compared with serum-fed cells. This treatment leads to increased expression of SMC differentiation markers such as ACTA2, MYH11 and TAGLN. We identified 31 significantly regulated miRNA candidates during this process, 28 rising and 3 falling. Human aortic SMCs (AoSMCs) were propagated in growth media. Control plates were harvested, and the remaining plates were serum starved for 48 (SS48) or 72 hours (SS72) in serum-free basal media. Several arrays were excluded from final analysis after QC, resulting in a final set of 10.

2009-12-25 | E-GEOD-19544 | biostudies-arrayexpress

RNA-Seq analysis of the retinal pigment epithelial cell line, ARPE19, during a time course of serum deprivation.

Project description:ARPE19 cells, derived from human retinal pigment epithelium (RPE), were cultured in serum supplemented media, then the serum was removed, and culture continued in serum free media. We use this culture system as a model for the early stages of Age-related macular degeneration. RNA-Seq was performed on RNAs from seven time points: day0 (serum supplemented), day1, 3, 4, 5, 6, and 9 serum deprived samples.

2020-05-13 | GSE129964 | GEO

Transcription profiling of human foreskin fibroblasts CRL 2091 (ATCC) serum starved for 48 hours, Serum Stimulation time series

Project description:Foreskin fibroblasts CRL 2091 (ATCC) were serum starved for 48 hours, and harvested at the indicated time points after switching to media with 10% FBS essentially as described (Iyer et al., 1999). RNA from all of the sampled time points were pooled as reference RNA to compare with RNA from individual time points as described (Iyer et al., 1999).

2005-10-31 | E-SMDB-2503 | biostudies-arrayexpress

Hypoxia serum starved Flt-1 wild type and Flt-1 heterozygote BMDMs

Project description:The goal is to identify differential gene expression between hypoxia serum starved (for 6 hours) Flt-1+/+ (wild type) and Flt-1+/- (VEGFR1 heterozygote) bone marrow derived macrophages

2019-06-30 | GSE116538 | GEO

Genome-wide analysis of serum starved prelamin A-accumulating hMSCs gene expression.

Project description:Analysis of serum starved prelamin A-accumulating hMSCs at gene expression level. The hypothesis tested in the present study was that prelamin A accumulation induces the dysregulation of genes that are essensial for cell survival under a stress condition such as serum starvation. The results provide important information about these genes and the functional categories that are dysregulated due to prelamin A accumulation in serum starved hMSCs. Two samples are analyzed in this microarray experiment: human mesenchymal stem cell cultured under serum starvation conditions (during 24 hours) which accumulate prelamin A (pre-hMSCs) and control mesenchymal stem cells (ctrl-hMSCs). 2 biological replicates (hMSCs derived from 2 different bone marrow donors) and 1 technical replicate are included in this analysis.

2014-06-23 | E-GEOD-52563 | biostudies-arrayexpress

LncRNA profile study during Human MSC adipogenic differentiation: Day0 vs Day3 vs Day6

Project description:LncRNA transcriptional profiling of human mesenchymal stem cells comparing control undifferentiated HMSC with Day3 and Day6 adipogenic differentiation stages Three-condition experiment, Day0 vs. Day3 vs Day6 cells. Day0: 4 replicates, Day3: 3 replicates and Day6: 3 replicates.

2014-05-22 | E-GEOD-57593 | biostudies-arrayexpress

Fibroblast secretome proteomic analysis

Project description:LV fibroblasts were isolated from control (day 0), and infarcted regions at day 1, day 3, and day 7 after myocardial infarction (MI). Cells were isolated in DMEM in 10% fetal bovine serum and were seeded into 6-well plates and were allowed to grow to 80% confluence in DMEM supplemented with 10% fetal bovine serum (FBS). Cells were serum starved (DMEM + 0.1% FBS) for 18 h and then media was changed and secretome was collected after 24 h. The sample sizes are n=3 biological replicates for each time point.

2019-10-25 | PXD011778 | Pride