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The biomolecular characterisation of an Early Neolithic finger ring from Syltholm, Denmark

ABSTRACT: We present the analysis of an osseous finger ring from an early Neolithic context in Denmark. To characterise the artefact and identify the raw material used for its manufacture, we performed micro-computed tomography (Micro CT) scanning, zooarchaeology by mass spectrometry (ZooMS) peptide mass fingerprinting, as well as protein sequencing by liquid chromatography tandem mass spectrometry (LC-MS/MS). We found that the ring was made from long bone or antler due to the presence of osteons (Haversian canals). Subsequent ZooMS analysis of the collagen present indicated that it was made from either elk (Alces alces) or red deer (Cervus elaphus) material. We then used LC-MS/MS analysis to refine our species identification, confirming that the ring was made from red deer, and to examine other proteins present. This study demonstrates the potential of ancient proteomics for species identification of prehistoric artefacts made from osseous material.

INSTRUMENT(S): orbitrap

ORGANISM(S): Cervus Elaphus (red Deer)

TISSUE(S): Bone, Antler

SUBMITTER: Meaghan Mackie

LAB HEAD: Hannes Schroeder

PROVIDER: PXD011811 | Pride | 2020-01-21

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180717_QE3_nLC3_MEM_COLLAB_GeoG_TJ_UofY_X2784.raw	Raw
	20190302_QE7_nLC7_MEM_COLLAB_TJ_UofY_P29.raw	Raw
	20190302_QE7_nLC7_MEM_COLLAB_TJ_UofY_P55.raw	Raw
	MQ-output-RingandModernReference.zip	Other
	MQoutput-Ring.zip	Other

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