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Proteomic analysis of TMV and TMV 24A+UPD infected N. benthamiana plants by iTRAQ reveal differences in stress signalling, cell death and response to intracellular iron

ABSTRACT: TMV was mutated by introducing different lengths of internal poly (A) tract in front of the upstream pseudo knotted domain .Twenty four nucleotides were inserted into the TMV genome, this mutant is designated TMV 24A +UPD TMV 24+UPD shows more severe leaf necrosis and stem collapse, leading to plant death within a few days. The difference in rate of virus replication and plant death may be because the poly (A) tract introduced into TMV may interact with UPD and affect its interaction with host factors. To understand TMV 24A+UPD interaction with the host and the events leading to the altered pathogenesis, we opted to evaluate its proteome profile and compare it to TMV using Isobaric tag for relative and absolute quantitation( iTRAQ).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Defu Yao

LAB HEAD: Prof. Wong Sek Man

PROVIDER: PXD011825 | Pride | 2019-10-17

REPOSITORIES: Pride

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			Action	DRS
	180502_Mecury_RPRP_8plex_5ul_5fractions_combinesearch.group	Other
	180502_Mecury_RPRP_8plex_5ul_5fractions_combinesearch.group.xml	Xml
	180502_Mecury_RPRP_8plex_5ul_5fractions_combinesearch__FDR.xlsx	Xlsx
	180502_Mecury_RPRP_8plex_F1_5ul-Mecury_RPRP_8plex_F1.mzML	Mzml
	180502_Mecury_RPRP_8plex_F1_5ul.wiff	Wiff

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