Browse
Submit Data
Databases
API
Help

Dataset Information

277 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ITRAQ based analysis of leaf proteome identifies important proteins in secondary metabolite biosynthesis and defence pathways crucial to cross-protection against TMV

ABSTRACT: We used milder strain of TMV mutant (TMV-43A) to cross protect Nicotiana benthamiana plants from TMV. We used iTRAQ (2 sets) quantitative proteomics approach to investigate molecular responses and identified important host proteins from secondary metabolite biosynthesis and defence pathways

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Nicotiana Benthamiana

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Prem Prakash Das

LAB HEAD: Wong Sek Man

PROVIDER: PXD011633 | Pride | 2018-11-21

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	171105_iTRAQset1_10fractions_5ul_1hr.group	Other
	171105_iTRAQset1_10fractions_5ul_1hr.group.xml	Xml
	171105_iTRAQset1_10fractions_5ul_1hr__FDR.xlsx	Xlsx
	171105_iTRAQset1_F10_5ul.mzML	Mzml
	171105_iTRAQset1_F10_5ul.wiff	Wiff

Items per page:

1 - 5 of 66

Similar Datasets

Differential regulation of tobacco host factors upon TMV infection

Project description:Tobacco mosaic virus (TMV) became one of the model virus for fundamental biological research and understanding of the host-pathogen interaction. It utilizes host machinery smartly to complete the life cycle, though many parts of life cycle remained a mystery, such as host factors involved in virus replication processes. We utilized the 8-Plex iTRAQ study to understand host-pathogen interaction between TMV and host tobacco.

2018-11-07 | PXD008837 | Pride

Proteomic analysis of TMV and TMV 24A+UPD infected N. benthamiana plants by iTRAQ reveal differences in stress signalling, cell death and response to intracellular iron

Project description:TMV was mutated by introducing different lengths of internal poly (A) tract in front of the upstream pseudo knotted domain .Twenty four nucleotides were inserted into the TMV genome, this mutant is designated TMV 24A +UPD TMV 24+UPD shows more severe leaf necrosis and stem collapse, leading to plant death within a few days. The difference in rate of virus replication and plant death may be because the poly (A) tract introduced into TMV may interact with UPD and affect its interaction with host factors. To understand TMV 24A+UPD interaction with the host and the events leading to the altered pathogenesis, we opted to evaluate its proteome profile and compare it to TMV using Isobaric tag for relative and absolute quantitation( iTRAQ).

2019-10-17 | PXD011825 | Pride

Arabidopsis protoplasts : TMV*rep vs. TMV*CP.MP transfected

Project description:Transcriptional profiling of Arabidopsis in response to infection with TMV in the initial infection stage (0.5, 4 and 6 hours post inoculation). Analyze the relative transcriptome change of TMV*CP.MP- and TMV*rep-inoculated samples. TMV*rep vs. TMV*CP.MP transfected cells. 2 biological replicates conducted at each time (0.5, 4 and 6 hours post inoculation)

2013-04-15 | E-GEOD-45283 | biostudies-arrayexpress

Arabidopsis protoplasts : TMV*rep vs. TMV*CP.MP transfected

2013-04-15 | GSE45283 | GEO

iTRAQ Manatee

Project description:In 2013, two large-scale Florida manatee (Trichechus manatus latirostris) mortality episodes were reported on separate coasts of Florida. The east coast mortality episode was associated with an unknown etiology in the Indian River Lagoon (IRL). The west coast mortality episode was attributed to a persistent Karenia brevis algal bloom or ‘red tide’ centered in Southwest Florida. To investigate these two mortality episodes, proteomic experiments using two-dimensional difference gel electrophoresis (2D-DIGE) followed by protein identification using liquid chromatography-tandem mass spectrometry (LC-MS/MS) were conducted, along with a separate gel-free analysis using isobaric tags for relative and absolute quantification (iTRAQ) LC-MS/MS. In comparison to the control group, manatees from the IRL, an area associated with an unknown mortality episode, displayed increased levels of several proteins in their serum samples. These increased proteins, which were identified in the iTRAQ experiment, included kininogen-1 isoform 1 (average ratio 1.38), protein AMBP (1.38), histidine-rich glycoprotein (1.34), properdin (1.30), and complement C4-A isoform 1 (1.25). In the red tide group, ceruloplasmin (2.32), pyruvate kinase isozymes M1/M2 isoform 3 (2.29), angiotensinogen (2.08), complement C4-A isoform 1 (1.83), and complement C3 (1.42) were increased. The proteins kininogen-1 isoform 1, histidine-rich glycoprotein, complement C4-A isoform 1, angiotensinogen, and complement C3 were also identified in increased levels in the 2D-DIGE experiment (Table 2b). These proteins are associated with acute-phase response, amyloid formation and accumulation, copper and iron homeostasis, the complement cascade pathway, and other important cellular functions. The increased level of complement C4 protein observed in both the red tide and unknown mortality episode groups was confirmed through the use of Western Blot.

2021-08-30 | PXD002470 | Pride

Tomato response to TMV after 1h of infection

Project description:TMV-resistant, TMV-susceptible and enhanced susceptibility mutant tomato were grown in asceptic conditions. At approximately 5 weeks, leaves were mock inoculated, TMV inoculated, or left untreated. Leaf tissue was collected 1hour after treatment. RNA was isolated and purified; hybridizations were performed using 130ug of total RNA from plants mock inoculated versus TMV inoculated and untreated. Each RNA sample is from two pooled plants. Two replicates were completed. Keywords: Direct comparison

2007-09-01 | GSE8206 | GEO

Tomato quantitative proteome - Post-transcriptional regulation of fruit ripening and disease resistance in tomato by the vacuolar protease SlVPE3

Project description:Identification of protease targets is essential to understand the molecular mechanism by which a protease regulates the biological processes. In this study, we applied an iTRAQ-based quantitative proteomic approach to identify the potential targets of a vacuolar processing enzyme in the cellular context in tomato. Proteins were isolated from fruits of wild-type and RNAi tomato plants. More than 300 proteins were finally screened as significantly altered in the RNAi fruits. Based on Blast2go, a web-based bioinformatic tool that categorizes proteins according to their Gene Ontology (GO) annotations, the proteins experienced significant changes in abundance in the RNAi fruits were classified into fifteen functional categories.

2017-02-20 | PXD002980 | Pride

iTRAQ in proteomic analysis of potential biomarkers in invasive cancer, ductal carcinoma in situ, and mammary fibroadenoma

Project description:Breast malignancy is a serious threat to women’s health around the world. However, for invasive, ductal carcinomas, and mammary fibroadenoma, there is an urgent demand for better breast malignancy-linked biomarkers. The current study was designed to identify diagnostic and/or therapeutic protein biomarkers for breast tumors. iTRAQ proteomic analysis was employed to characterize differentially expressed protein biomarkers. We identified several potential protein biomarkers that can be recommended for further testing as diagnostic and/or therapeutic targets in breast tumors.

2020-10-13 | PXD019963 | Pride

Profiling of genes related to cross protection and competition for NbTOM1 by HLSV and TMV

Project description:To investigate the mechanism under the cross protection between HLSV and TMV, microarray analysis was conducted to examine the transcriptional levels of global host genes during cross protection, using Tobacco Gene Expression Microarray, 4x44k slides. The transcriptional level of some host genes corresponded to accumulation level of TMV. Some host genes were up-regulated only by HLSV. The gene expression in Nicotiana benthamiana during cross protection was measured at 12 days post HLSV or mock buffer inoculated as well as TMV infected N. benthamiana which pre-inoculated HLSV or mock bufer at 3 dpi (which is equal to 15 dpi by HLSV).

2014-05-12 | E-GEOD-47180 | biostudies-arrayexpress

Identify Novel Iron–Transporting Proteins in Streptococcus pneumoniae

Project description:Streptococcus pneumoniae (S. pneumoniae) is a major human pathogen causing morbidity and mortality worldwide. Efficiently acquiring iron from the environment is critical for S. pneumoniae to sustain growth and cause infection. There are only three known iron-uptake systems in Streptococcal species responsible for iron acquisition from the host, including ABC transporters PiaABC, PiuABC and PitABC. Besides, no other iron-transporting system has been suggested. In this work, we employed our newly established translating mRNA analysis integrated with proteomics to evaluate the possible existence of novel iron transporters in the bacterium. We simultaneously deleted the iron-binding protein genes of the three iron-uptake systems to construct a piaA/piuA/pitA triple mutant (Tri-Mut) of S. pneumoniae D39, in which genes and proteins related to iron transport should be regulated in response to the deletion. With ribosome associated mRNA sequencing-based translatomics focusing on translating mRNA and iTRAQ quantitative proteomics based on the covalent labeling of peptides with tags of varying mass, we indeed observed a large number of genes and proteins representing various coordinated biological pathways with significantly altered expression levels in the Tri-Mut mutant. Highlighted in this observation is the identification of several new potential iron-uptake ABC transporters for Streptococcal iron metabolism. In particular, putative protein SPD_1609 in operon 804 was verified to be a novel iron-binding protein with similar function to PitA in S. pneumoniae. These data derived from the integrative translatomics and proteomics analyses provided rich information and insightful clues for further investigations on iron-transporting mechanism in bacteria and the interplay between Streptococcal iron availability and the biological metabolic pathways.

2018-10-26 | PXD003313 | Pride