Proteomics profiling of the cell cycle provides novel insight in dynamics of protein turnover, phosphorylation and subcellular localization.
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
SUBMITTER: 
Gianluca Maddalo
LAB HEAD: Mathias Uhlen
PROVIDER: PXD011836 | Pride | 2020-02-18
REPOSITORIES: Pride
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Publications
Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics.
Herr Patrick P Boström Johan J Rullman Eric E Rudd Sean G SG Vesterlund Mattias M Lehtiö Janne J Helleday Thomas T Maddalo Gianluca G Altun Mikael M
Molecular & cellular proteomics : MCP 20200212 4
The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISP ...[more]
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