Dataset Information

Quantitative proteomics of melanoma metastases derived from adherent cells or suspended cells.

ABSTRACT: Detachment of melanoma and survival under anchorage-independency were recognized as initial step of tumor metastasis. Our previous studies showed altered gene expression contributed to loss of cell invasiveness, enhanced chemosensitivity, and enhanced subcutaneous tumor formation. In this study, we demonstrated that melanoma cell detachment altered vascular phenotype of melanoma metastases through disruption of expression axis of aminopeptidase-N/syndecan-1/integrin beta4, which would contribute to melanoma heterogeneity.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Wei-Chi Ku

LAB HEAD: Shao-Chen Lee

PROVIDER: PXD011883 | Pride | 2020-08-07

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	2013-12-17-yiling10-nuclear-CAPA-30mM-2.raw	Raw
	2013-12-17-yiling11-nuclear-CAPA-30mM-3.raw	Raw
	2013-12-17-yiling12-nuclear-CAPA-60mM-1.raw	Raw
	2013-12-17-yiling13-nuclear-CAPA-60mM-2.raw	Raw
	2013-12-17-yiling14-nuclear-CAPA-60mM-3.raw	Raw

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Publications

Anchorage independence altered vasculogenic phenotype of melanoma cells through downregulation in aminopeptidase N /syndecan-1/integrin β4 axis.

Cheng Yu-Che YC Ku Wei-Chi WC Tseng Ting-Ting TT Wu Ching-Po CP Li Mengjin M Lee Shao-Chen SC

Aging 20200804 17

The detachment of tumor cells from extracellular matrix and survival under anchorage-independence were recognized as the initial step of tumor metastasis. Previously we had demonstrated that anchorage-independence altered gene expressions and showed characteristics of cell invasiveness loss, enhanced chemosensitivity, and enhanced subcutaneous tumor formation. However, whether it affected histological phenotypes in tumor tissues remained unclear. Melanoma metastases were generated in nude mice u ...[more]

PMID: 32756007

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