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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of mouse non-metastatic and metastatic melanoma cells with and without 5AzaCdR treatment.

ABSTRACT: Comparison of melanoma cell lineages responses to 5AzaCdR with control

ORGANISM(S): Mus musculus

DISEASE(S): non-metastatic melanoma

SUBMITTER: Miriam Jasiulionis

PROVIDER: E-MEXP-2517 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of mouse non-tumorigenic melanocytes with and without 5AzaCdR treatment.

Project description:Comparison of non-tumorigenic melanocytes responses to 5AzaCdR with control

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Expression data from UKRV-Mel-15a melanoma-derived clones resistant to cytolysis mediated by Melan-A/MART1 (26-35) cytotoxic T lymphocytes (CTL).

Project description:Efficient processing of target antigens by the ubiquitin-proteasome-system (UPS) is essential for treatment of cancers by T cell therapies. However, immune escape due to impaired expression of IFN-Î³-inducible components of the antigen presentation machinery and consequent inefficient processing of HLA-dependent tumor epitopes can be one important reason for failure of such therapies. Here, we show that repeated short-term co-cultures of Melan-A/MART-1 tumor antigen-expressing melanoma cells with Melan-A/MART-1 (26-35)-specific CTL led to the generation of clones resistant to CTL-mediated cell death. To determine which of the UPS components and its associated pathways was responsible for CTL escape; three UKRV-Mel-15a clones were subjected to microarray gene expression analysis. Three UKRV-Mel-15a-derived melanoma clones were isolated following three repeated short-term exposures to Melan-A/MART (26-35) CTL and harvested for RNA extraction and hybridization on Affymetrix microarrays.

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Transcription profiling of maize to identify gene expression changes after chilling for 4 hours (alarm phase)

Project description:This experiment evaluates quick (alarm) response to chilling in chilling-sensitive maize plants. Maize inbred line cm109 were grown in optimal conditions until third leaf was fully developed. At this stage plants were divided into three experimental variants: k0 - control plants, frozen at the beginning of daylight, k4 - control plants kept in the same conditions and frozen after 4 hours since beginning of daylight, c4 - plants kept in 14 deg. C for 4 hours since "dawn". At the mentioned moments, leaves were harvested and frozen in liquid nitrogen for RNA isolation.

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RNA immunoprecipitation of Trypanosoma cruzi to study messenger ribonucleoproteins (mRNPs) containing TcDhh1.

Project description:To study the composition of mRNPs containing TcDhh1, we carried out immunoprecipitation assays with anti-TcDhh1 and epimastigotes lysates. Pre-immune serum was used as control. We also carried out a ribonomic approach to identify the mRNAs present within the TcDhh1 immunoprecipitated complexes. For this purpose, competitive microarray hibridizations were performed against negative controls, the non-precipitated fraction.

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Transcription profiling of rat heart transplants from Lewis to Lewis and Lewis to F344 strains with and without cold storage / prolonged cold ischemia.

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Transcription profiling of human embryonic kidney cell line HEK293T to investigate the effects of overexpression of FoxO4 or mutated FoxO4 in presence or absence of p300

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