Dataset Information

Meltome Atlas - thermal proteome stability across the tree of life

ABSTRACT: We have developed a mass spectrometry-based approach that allowed us to quantitatively monitor protein stability across a broad range of temperatures at the proteome scale (meltome). We profiled the meltomes of several microorganisms and eukaryotic species including human, allowing to investigate the determinants of protein thermostability and survival in various environmental niches. Moreover, we will make Meltome Atlas a valuable, publicly available resource for the biological community to investigate their proteins of interest in the context of protein thermostbility.

INSTRUMENT(S): Orbitrap Fusion Lumos, Exactive Plus

ORGANISM(S): Thermus Thermophilus (strain Hb8 / Atcc 27634 / Dsm 579) Picrophilus Torridus Oleispira Antarctica Danio Rerio (zebrafish) (brachydanio Rerio) Caenorhabditis Elegans Saccharomyces Cerevisiae (baker's Yeast) Bacillus Subtilis Drosophila Melanogaster (fruit Fly) Homo Sapiens (human) Escherichia Coli Arabidopsis Thaliana (mouse-ear Cress) Geobacillus Stearothermophilus Mus Musculus (mouse)

TISSUE(S): Hepatocyte, Blood Plasma, Seminal Plasma, Liver, Cerebrospinal Fluid, Fibroblast Of Lung, Fibroblast, Saliva, Milk

SUBMITTER: Anna Jarzab

LAB HEAD: Bernhard Kuster

PROVIDER: PXD011929 | Pride | 2020-04-29

REPOSITORIES: Pride

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			Action	DRS
	00820_F1_R1_P0100960B02_TMT10.raw.rar	Raw
	00820_F1_R1_P0100960B03_TMT10.raw.rar	Raw
	00820_F1_R1_P0100960B04_TMT10.raw.rar	Raw
	00820_F1_R1_P0100960B05_TMT10.raw.rar	Raw
	00820_F1_R1_P0100960B06_TMT10.raw.rar	Raw

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Publications

Meltome atlas-thermal proteome stability across the tree of life.

Jarzab Anna A Kurzawa Nils N Hopf Thomas T Moerch Matthias M Zecha Jana J Leijten Niels N Bian Yangyang Y Musiol Eva E Maschberger Melanie M Stoehr Gabriele G Becher Isabelle I Daly Charlotte C Samaras Patroklos P Mergner Julia J Spanier Britta B Angelov Angel A Werner Thilo T Bantscheff Marcus M Wilhelm Mathias M Klingenspor Martin M Lemeer Simone S Liebl Wolfgang W Hahne Hannes H Savitski Mikhail M MM Kuster Bernhard B

Nature methods 20200413 5

We have used a mass spectrometry-based proteomic approach to compile an atlas of the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans and covering melting temperatures of 30-90 °C. Protein sequence, composition and size affect thermal stability in prokaryotes and eukaryotic proteins show a nonlinear relationship between the degree of disordered protein structure and thermal stability. The data indicate that evolutionary conservation of protein complexes is re ...[more]

PMID: 32284610

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