Dataset Information

2D gel-based proteomics of primary human peritoneal mesothelial cells derived from omentum

ABSTRACT: Peritoneal mesothelial cells are harmed by peritoneal dialysis fluids (PDF) used in renal replacement therapy with peritoneal dialysis. The mechanisms of the cellular damage are not yet described in detail. Primary human peritoneal mesothelial cells derived from omentum of five donors were independently exposed to peritoneal dialysis fluids. The extent of cell damage was assessed using lactate dehydrogenase (LDH) release in the cell culture supernatant and cells were lysed in order to extract mRNA and proteins. Transcriptional changes induced by PDF were analyzed using gene expression microarrays and changes of the proteome were analyzed using 2D-electrophoresis.

INSTRUMENT(S): MALDI LTQ Orbitrap

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Primary Cell

SUBMITTER: Klaus Kratochwill

LAB HEAD: Dr Klaus Kratochwill

PROVIDER: PXD011938 | Pride | 2022-02-22

REPOSITORIES: Pride

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Lithium preserves peritoneal membrane integrity by suppressing mesothelial cell αB-crystallin.

Herzog Rebecca R Sacnun Juan Manuel JM González-Mateo Guadalupe G Bartosova Maria M Bialas Katarzyna K Wagner Anja A Unterwurzacher Markus M Sobieszek Isabel J IJ Daniel-Fischer Lisa L Rusai Krisztina K Pascual-Antón Lucía L Kaczirek Klaus K Vychytil Andreas A Schmitt Claus Peter CP López-Cabrera Manuel M Alper Seth L SL Aufricht Christoph C Kratochwill Klaus K

Science translational medicine 20210801 608

Life-saving renal replacement therapy by peritoneal dialysis (PD) is limited in use and duration by progressive impairment of peritoneal membrane integrity and homeostasis. Preservation of peritoneal membrane integrity during chronic PD remains an urgent but long unmet medical need. PD therapy failure results from peritoneal fibrosis and angiogenesis caused by hypertonic PD fluid (PDF)-induced mesothelial cytotoxicity. However, the pathophysiological mechanisms involved are incompletely understo ...[more]

PMID: 34433641

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