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The relative intensity of RhPIP2;1 at Ser 273. Membrane proteins were extracted from mock (left) and dehydrated (right) leaves and were used for LC-MS analysis - Part 2

ABSTRACT: Plants have evolved sophisticated systems to respond to environmental changes, and growth arrest is a common strategy to enhance stress tolerance. Despite the growth-survival tradeoffs is essential for shaping plant productivity, the mechanisms balancing growth and survival remain largely unknown. Aquaporins play a crucial role in growth and stress responses by controlling water transport across membranes. Here, we present that RhPIP2;1, an aquaporin from rose (Rosa sp.), interacts with a membrane-tethered MYB protein, RhPTM. Water deficiency triggers nuclear translocation of RhPTM C-terminus. Silencing of RhPTM causes continuous growth under drought stress, and a consequent decrease in survival rate. RNA-seq indicates that RhPTM influences expression of genes related to carbohydrate metabolism. Water deficiency induces phosphorylation of RhPIP2;1 at Ser273, which is sufficient to promote nuclear translocation of RhPTM C-terminus. These results indicate that RhPIP2;1-RhPTM module serves as a critical player to orchestrate the tradeoff between growth and stress survival in rose.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rosa Hybrid Cultivar

TISSUE(S): Leaf

SUBMITTER: shuai zhang

LAB HEAD: Junping Gao

PROVIDER: PXD011943 | Pride | 2019-01-16

REPOSITORIES: Pride

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			Action	DRS
	RhPTM.raw	Raw
	RhPTM.xls	Xls
	RhPTMCEND.raw	Raw
	RhPTMCEND.xml	Xml

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The relative intensity of RhPIP2;1 at Ser 273. Membrane proteins were extracted from mock (left) and dehydrated (right) leaves and were used for LC-MS analysis - Part 1

Project description:Plants have evolved sophisticated systems to respond to environmental changes, and growth arrest is a common strategy to enhance stress tolerance. Despite the growth-survival tradeoffs is essential for shaping plant productivity, the mechanisms balancing growth and survival remain largely unknown. Aquaporins play a crucial role in growth and stress responses by controlling water transport across membranes. Here, we present that RhPIP2;1, an aquaporin from rose (Rosa sp.), interacts with a membrane-tethered MYB protein, RhPTM. Water deficiency triggers nuclear translocation of RhPTM C-terminus. Silencing of RhPTM causes continuous growth under drought stress, and a consequent decrease in survival rate. RNA-seq indicates that RhPTM influences expression of genes related to carbohydrate metabolism. Water deficiency induces phosphorylation of RhPIP2;1 at Ser273, which is sufficient to promote nuclear translocation of RhPTM C-terminus. These results indicate that RhPIP2;1-RhPTM module serves as a critical player to orchestrate the tradeoff between growth and stress survival in rose.

2019-01-16 | PXD011942 | Pride

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Project description:Water deficit stress (WDS) is a crucial factor that causes the inhibition of petal expansion and abnormal flower opening in rose. The regulatory mechanisms of petal expansion by WDS at transcriptional level were investigated by analysis expression profiles under WDS. Analysis used total RNA samples of petals taken from flowers treated by WDS comparison to those from control flowers. Transcriptome dynamics during treatment time responsive to WDS.

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Analysis of the effect of exogenous auxin on the regulatory relationship between miRNA and its target genes in rose using high-throughput sequencing [RNA-seq]

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Project description:BACKGROUND: The ability to adapt to environments with fluctuating nutrient availability is vital for bacterial survival. Although essential for growth, few nitrogen metabolism genes have been identified or fully characterised in mycobacteria and nitrogen stress survival mechanisms are unknown. RESULTS: A global transcriptional analysis of the mycobacterial response to nitrogen stress, showed a significant change in the differential expression of 16% of the Mycobacterium smegmatis genome. Gene expression changes were mapped onto the metabolic network using Active Modules for Bipartite Networks (AMBIENT) to identify metabolic pathways showing coordinated transcriptional responses to the stress. AMBIENT revealed several key features of the metabolic response not identified by KEGG enrichment alone. Down regulated reactions were associated with the general reduction in cellular metabolism as a consequence of reduced growth rate. Up-regulated modules highlighted metabolic changes in nitrogen assimilation and scavenging, as well as reactions involved in hydrogen peroxide metabolism, carbon scavenging and energy generation. CONCLUSIONS: Application of an Active Modules algorithm to transcriptomic data identified key metabolic reactions and pathways altered in response to nitrogen stress, which are central to survival under nitrogen limiting environments. [Data is also available from http://bugs.sgul.ac.uk/E-BUGS-140]

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