Dataset Information

Discovery and structure of the mitoribosomal SSU assemblosome in trypanosomes

ABSTRACT: Data-dependent LC-MS/MS was used to generate a protein inventory of an affinity-purified assembly intermediate of the small subunit (SSU) of the Trypanosoma brucei mitochondrial ribosome. The identified proteins (mitoribosomal proteins and putative maturation factors) were then used to assist in solving the structure of the SSU assemblosome by cryo-electron microscopy.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Trypanosoma Brucei Brucei Treu927

SUBMITTER: Alexander Leitner

LAB HEAD: Nenad Ban

PROVIDER: PXD011988 | Pride | 2019-09-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	aleitner_C1706_269.c.mgf	Mgf
	aleitner_C1706_269.c.pride.mgf.gz	Mgf
	aleitner_C1706_269.mzid.gz	Mzid
	aleitner_C1706_269.pride.mztab.gz	Mztab
	aleitner_C1706_269.raw	Raw

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Publications

Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.

Saurer Martin M Ramrath David J F DJF Niemann Moritz M Calderaro Salvatore S Prange Céline C Mattei Simone S Scaiola Alain A Leitner Alexander A Bieri Philipp P Horn Elke K EK Leibundgut Marc M Boehringer Daniel D Schneider André A Ban Nenad N

Science (New York, N.Y.) 20190901 6458

Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in <i>Trypanosoma brucei</i> involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron ...[more]

PMID: 31515389

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