Dataset Information

Proteome Dataset for Adult-derived Mouse Microglia

ABSTRACT: We report methods development for quantitative proteomic analysis of samples with limited number of cells. The improvements include suspension trapping (S-Trap) for sample processing, longer analytical column length (75cm), and a segmented data dependent acquisition approach. The modified approach was applied to the proteomic analysis of adult-derived mouse microglia and optimized to the cell count expected from mouse brain (~300K microglial cells). With the improved approach, over 4500 quantifiable proteins can be obtained (in biological triplicate) with an average CV of 10%.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Microglial Cell

SUBMITTER: Stanley Stevens

LAB HEAD: Stanley M. Stevens, Jr.

PROVIDER: PXD012006 | Pride | 2019-04-18

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20180724_70KSDDA15_STrap_300k_1.raw	Raw
	20180724_70KSDDA15_STrap_300k_2.raw	Raw
	20180724_70KSDDA15_STrap_300k_3.raw	Raw
	20180724_DDA15_STrap_300k_1.raw	Raw
	20180724_DDA15_STrap_300k_2.raw	Raw

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Publications

Improved Methodology for Sensitive and Rapid Quantitative Proteomic Analysis of Adult-Derived Mouse Microglia: Application to a Novel In Vitro Mouse Microglial Cell Model.

Guergues Jennifer J Zhang Ping P Liu Bin B Stevens Stanley M SM

Proteomics 20190509 11

Microglia, as the resident brain immune cells, can exhibit a broad range of activation phenotypes, which have been implicated in a multitude of central nervous system disorders. Current widely studied microglial cell lines are mainly derived from neonatal rodent brain that can limit their relevance to homeostatic function and disease-related neuroimmune responses in the adult brain. Recently, an adult mouse brain-derived microglial cell line has been established; however, a comprehensive proteom ...[more]

PMID: 30980500

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