Dataset Information

The FGF4-dependent phosphoproteome in mouse embryonic stem cells

ABSTRACT: Protein kinase signalling is a major mechanism by which embryonic stem cell pluripotency and differentiation is controlled. However, the pathways and components that regulate embryonic stem cell identity have not been systematically defined. Here, we employ FGF4 signalling as a model system to investigate phosphoproteome dynamics in differentiating mouse embryonic stem cells. We report identification and quantitation of more than 10,000 phosphopeptides, of which hundreds of phosphophoylation sites are regulated more than 2-fold by acute FGF4 stimulation. We hypothesise that phosphorylation sites in this dataset are relevant for regulating the transition of mouse embryonic stem cells from pluripotency towards lineage specific differentiation.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Houjiang Zhou

LAB HEAD: Greg Findlay

PROVIDER: PXD012069 | Pride | 2020-02-24

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	20180930_HZ_GF_EPHA_TMT9plex_F1.raw	Raw
	20180930_HZ_GF_EPHA_TMT9plex_F10.raw	Raw
	20180930_HZ_GF_EPHA_TMT9plex_F11.raw	Raw
	20180930_HZ_GF_EPHA_TMT9plex_F12.raw	Raw
	20180930_HZ_GF_EPHA_TMT9plex_F13.raw	Raw

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Publications

Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation.

Fernandez-Alonso Rosalia R Bustos Francisco F Budzyk Manon M Kumar Pankaj P Helbig Andreas O AO Hukelmann Jens J Lamond Angus I AI Lanner Fredrik F Zhou Houjiang H Petsalaki Evangelia E Findlay Greg M GM

Nature communications 20200313 1

Embryonic Stem Cell (ESC) differentiation requires complex cell signalling network dynamics, although the key molecular events remain poorly understood. Here, we use phosphoproteomics to identify an FGF4-mediated phosphorylation switch centred upon the key Ephrin receptor EPHA2 in differentiating ESCs. We show that EPHA2 maintains pluripotency and restrains commitment by antagonising ERK1/2 signalling. Upon ESC differentiation, FGF4 utilises a bimodal strategy to disable EPHA2, which is accompan ...[more]

PMID: 32170114

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