Dataset Information

Wnt-modulated hiPSC-derived insulin-producing cells, adult human islet proteome

ABSTRACT: We aimed to assess whether Wnt-modulation could contribute to mature hiPSC-derived insulin-producing cells in vitro. Building our hypothesis on our previous findings of Wnt activation in immature hiPSC-derived insulin-producing cells compared to adult human islets and with recent data reporting a link between Wnt/PCP and in vitro beta-cell maturation. In this study we stimulated hiPSC-derived insulin-producing cells with syntetic proteins including WNT3A, WNT4, WNT5A and WNT5B as well as inhibiting endogeneous Wnt signaling with Tankyrase inhibitor G007-LK.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Islet Of Langerhans, Cell Culture

SUBMITTER: Heidrun Vethe

LAB HEAD: Helge Ræder

PROVIDER: PXD012081 | Pride | 2019-11-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	271328_a04017_HV_wt.mzIdentML	Mzid
	271329_a04018_HV_wt.mzIdentML	Mzid
	271330_a04019_HV_wt.mzIdentML	Mzid
	271331_a04020_HV_wt.mzIdentML	Mzid
	271332_a04021_HV_wt.mzIdentML	Mzid

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Publications

The Effect of Wnt Pathway Modulators on Human iPSC-Derived Pancreatic Beta Cell Maturation.

Vethe Heidrun H Ghila Luiza L Berle Magnus M Hoareau Laurence L Haaland Øystein A ØA Scholz Hanne H Paulo Joao A JA Chera Simona S Ræder Helge H

Frontiers in endocrinology 20190508

Current published protocols for targeted differentiation of human stem cells toward pancreatic β-cells fail to deliver sufficiently mature cells with functional properties comparable to human islet β-cells. We aimed to assess whether Wnt-modulation could promote the final protocol stages of β-cell maturation, building our hypothesis on our previous findings of Wnt activation in immature hiPSC-derived stage 7 (S7) cells compared to adult human islets and with recent data reporting a link between ...[more]

PMID: 31139151

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