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LC-MS/MS analysis of an intact synthetic microRNA

ABSTRACT: The field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgun proteomics. We have developed a free and open-source database search engine for RNA MS data, called NucleicAcidSearchEngine (NASE), as part of the OpenMS software framework. NASE allows the reliable identification of (modified) RNA sequences from LC-MS/MS data in a high-throughput fashion. For this validation dataset, oligonucleotides with the sequence of mature Drosophila let-7 microRNA, 21 nt in length, were produced synthetically in unmodified and modified (2’-O-methylated at the 3’ uridine) forms. We characterised a 1:1 mixture of both forms of this RNA.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Hendrik Weisser

LAB HEAD: Byron Andrews

PROVIDER: PXD012094 | Pride | 2019-01-10

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	Synthetic_let7.idXML	Other
	Synthetic_let7.mzML	Mzml
	Synthetic_let7.mzTabNA	Mztab
	let7.fasta	Fasta

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