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LC-MS/MS analysis of human cellular tRNA

ABSTRACT: The field of epitranscriptomics is growing in importance, with chemical modification of RNA being associated with a wide variety of biological phenomena. Mass spectrometry (MS) enables the identification of modified RNA residues within their sequence contexts, by using analogous approaches to shotgun proteomics. We have developed a free and open-source database search engine for RNA MS data, called NucleicAcidSearchEngine (NASE), as part of the OpenMS software framework. NASE allows the reliable identification of (modified) RNA sequences from LC-MS/MS data in a high-throughput fashion. For this validation dataset, we generated a sample of human total tRNA from a cellular extract - a complex mixture of highly modified RNAs. This sample was RNase-treated prior to nanoflow LC-MS/MS analysis.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Hendrik Weisser

LAB HEAD: Byron Andrews

PROVIDER: PXD012097 | Pride | 2018-12-31

REPOSITORIES: Pride

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			Action	DRS
	HAP1_tRNA.mzML.gz	Mzml
	HAP1_tRNA_FDR10.idXML	Other
	HAP1_tRNA_FDR10.mzTabNA	Mztab
	hg19-mature-tRNA_TargetDecoy.fasta	Fasta

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LC-MS/MS analysis of human cellular tRNA

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