Dataset Information

RUNX2 stimulates neoangiogenesis through the RUNT domain in melanoma

ABSTRACT: Transcription factors, by acting on gene expression can affect molecular pathways and can thereby induce malignant transformation. Among the transcription factors involved in cellular transformation, we recently highlighted the role of RUNX2 in melanoma cell migration and proliferation. In the present study, we extended the analysis of the molecular effects of RUNT domain of RUNX2 in melanoma cells to deepen understanding of the underlying mechanisms. By the CRISPR/Cas9 system we generated the RUNT KO melanoma cells 3G8. Therefore, using a proteomic approach, we compared 3G8 with the original wild-type melanoma cell line (A375). The results showed a specific protein signature of 3G8 cells related to apoptosis and migration. In addition, proteomic analysis allowed us to point out the involvement of the RUNT domain in the neoangiogenesis process. Among the deregulated proteins implicated in angiogenesis we found fatty acid synthase, chloride intracellular channel protein-4, heat shock protein beta-1, Rho guanine nucleotide exchange factor 1, D-3-phosphoglycerate dehydrogenase, myosin-1c and caveolin-1. Accordingly, the VEGF gene expression was higher in A375 compared to 3G8 cells; HUVEC cells expressed increased levels of the neoangiogenetic markers CD105 and CD31 when co-cultured with A375 cells. In conclusion, our study provided new insight into RUNX2 molecular details which can be crucial to possibly propose it as an oncotarget of melanoma.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Marcello Manfredi

LAB HEAD: Marcello Manfredi

PROVIDER: PXD012096 | Pride | 2019-11-13

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	LisatiVerona_3G8_1_1ug_09082017_LIB1.dat	Other
	LisatiVerona_3G8_1_1ug_09082017_LIB1.mgf	Mgf
	LisatiVerona_3G8_1_2ug_09082017_LIB1.dat	Other
	LisatiVerona_3G8_1_2ug_09082017_LIB1.mgf	Mgf
	LisatiVerona_3G8_1_2ug_09082017_LIB2.dat	Other

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Runx2 stimulates neoangiogenesis through the Runt domain in melanoma.

Cecconi Daniela D Brandi Jessica J Manfredi Marcello M Serena Michela M Dalle Carbonare Luca L Deiana Michela M Cheri Samuele S Parolini Francesca F Gandini Alberto A Marchetto Giulia G Innamorati Giulio G Avanzi Francesco F Antoniazzi Franco F Marengo Emilio E Tiso Natascia N Mottes Monica M Zipeto Donato D Valenti Maria Teresa MT

Scientific reports 20190529 1

Runx2 is a transcription factor involved in melanoma cell migration and proliferation. Here, we extended the analysis of Runt domain of Runx2 in melanoma cells to deepen understanding of the underlying mechanisms. By the CRISPR/Cas9 system we generated the Runt KO melanoma cells 3G8. Interestingly, the proteome analysis showed a specific protein signature of 3G8 cells related to apoptosis and migration, and pointed out the involvement of Runt domain in the neoangiogenesis process. Among the prot ...[more]

PMID: 31142788

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