Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome Analysis of Arabidopsis thaliana PP7L mutants

ABSTRACT: Chloroplast biogenesis is indispensable for proper plant development. In a screen for photosynthesis affected mutants, we have identified the pp7l (serine/threonine-protein phosphatase7-like) mutant in which chloroplast development is delayed in cotyledons and young leaves. PP7L constitutes together with PP7 and PP7-long the type 7 subfamily of serine/threonine-specific phosphoprotein phosphatases (PPPs). Here we performed shotgun proteomic experiment in order to profile the changes in protein levels in the pp7l mutants in comparison to the wild-type (Col-0).

OTHER RELATED OMICS DATASETS IN: GSE122495

INSTRUMENT(S): impact II

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seedling

SUBMITTER: Giada Marino

LAB HEAD: Tatjana Kleine

PROVIDER: PXD012105 | Pride | 2019-02-19

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	MaxQuant_Output.zip	Other
	Raw-data-pp7l.zip	Other

Items per page:

1 - 2 of 2

Publications

Extrachloroplastic PP7L Functions in Chloroplast Development and Abiotic Stress Tolerance.

Xu Duorong D Marino Giada G Klingl Andreas A Enderle Beatrix B Monte Elena E Kurth Joachim J Hiltbrunner Andreas A Leister Dario D Kleine Tatjana T

Plant physiology 20190213 1

Chloroplast biogenesis is indispensable for proper plant development and environmental acclimation. In a screen for mutants affected in photosynthesis, we identified the protein phosphatase7-like (pp7l) mutant, which displayed delayed chloroplast development in cotyledons and young leaves. PP7L, PP7, and PP7-long constitute a subfamily of phosphoprotein phosphatases. PP7 is thought to transduce a blue-light signal perceived by crys and phy a that induces expression of ...[more]

PMID: 30760637

Similar Datasets

E-GEOD-122495

Project description:SERINE/THREONINE-PROTEIN PHOSPHATASE7-LIKE PP7L Regulates Chloroplast Development

2020-04-16 | E-GEOD-122495 | ExpressionAtlas

SERINE/THREONINE-PROTEIN PHOSPHATASE7-LIKE PP7L Regulates Chloroplast Development

Project description:SERINE/THREONINE-PROTEIN PHOSPHATASE7-LIKE PP7L Regulates Chloroplast Development

| PRJNA505350 | ENA

JHI Hv GC whirly

Project description:The WHIRLY1 protein, which binds to single stranded DNA in plastids and the nucleus, is involved in chloroplast to nucleus signaling but its precise functions remain to be characterized. Here we investigated WHIRLY1 functions in photosynthesis and nitrogen metabolism using transgenic barley lines (W1-1, W1-7 and W1-9) with less than 2% of the wild type WHIRLY1 protein. The WHIRLY1-deficient lines had significantly more leaf chlorophyll with lower chlorophyll a/b ratios and less protein than the wild type when grown with either 5mM KNO3 (nitrogen replete) or 0.1mM KNO3 (nitrogen deficient). Relatively few leaf transcripts were changed in abundance as a result of WHIRLY1 deficiency but a small suite of chloroplast mRNAs was decreased including transcripts encoding proteins of the the NAD(P)H dehydrogenase (NDH) complex. Photosynthesis, stomatal conductance and transpiration were changed in W1-7 leaves compared to the wild type. The W1-7 leaves had significantly less glutamine, threonine, methionine, serine, succinate, fumarate, malate and citrate, as well as decreased transcripts encoding key enzymes of chloroplast amino acid biosynthesis, particularly the branched chain and shikimate pathways. These data demonstrate that Whirly 1 fulfils crucial roles in the regulation of photosynthesis and associated processes such as chloroplast amino acid synthesis

2014-10-01 | E-MTAB-2242 | biostudies-arrayexpress

Deciphering the Protein-Protein Interaction Network Regulating Hepatocelluler Carcinoma Metastasis

Project description:Hepatocellular carcinoma (HCC) is one of the leading causes of mortality related to cancer all over the world. The poor prognosis of HCC is mostly due to recurrence and tumor metastasis. In order to better understand the molecular mechanisms of HCC metastasis, we analyzed the proteome of three HCC cell lines with different metastasis potentials by using quantitative proteomics and bioinformatics analysis. As a result, we identified 331 cellular proteins potentially associated to HCC metastasis, and constructed a highly connected protein-protein interaction (PPI) network. Functional annotation of the network uncovered prominent pathways and key roles of these proteins, suggesting that metabolism and cytoskeleton biological progresses are greatly involved with HCC metastasis. Furthermore, integrative network analysis revealed a rich-club organization in the PPI network indicating a hub center of connections, including several well-known cancer related proteins, such as SRC proto-oncogene, non-receptor tyrosine kinase (SRC) and pyruvate kinase M2 (PKM2). Moreover, the differential expressions of two identified proteins, including PKM2 and actin-related protein 2/3 complex subunit 4 (ARPC4), were validated using Western blotting. These two proteins were identified as potential prognostic markers for HCC by using survival rate analysis.

2017-06-20 | PXD005647 | Pride

Gene expression of Streptococcus mutans pknB mutant versus wild type strain

Project description:Bacteria can detect, transmit and react to signals from the outside world by using two-component systems an serine-threonine kinases and phosphatases. Streptococcus mutans contains one serine-threonine kinase, encoded by pknB. A gene encoding a serine-threonine phosphatase, pppL, is located downstream of pknB. In this study, the phenotypes of single mutants in pknB and pppL and a pknBpppL double mutant were characterized. All mutants exhibited a reduction in genetic transformability and biofilm formation, showed abnormal cell shapes, grew slower than the wild type strain in several complex media and had lost acid tolerance. The mutants had reduced cariogenic capacity, but no defects in colonization in a rat caries model. Whole genome transcriptome analysis revealed that pknB mutant showed reduced expression of genes involved in bacteriocin production and genetic competence. Among the genes that were diferentially regulated in the pknB mutant, severeal were likely to be involved in cell wall metabolism. One such gene, SMU.2146c and two genes encoding bacteriocins, were showed to be also down-regulated in vicK, which encodes a sensor kinase involved in response to oxidative stress. Collectively, the results suggest that PknB can modulate the activity of the two-component signal transduction systems vicKR and comDE. Real-time PCR showed that the genes down-regulated in the pknB mutant were up-regulated in the pppL mutant, indicating that PppL served to counteract PknB. Wild-type strain UA159 and a pknB mutant derivative were grown planktonically until OD600nm was 0.3. RNA was extracted, converted to cDNA, labelled and hybridized on S. mutans microarrays. The experiment was done in triplicate including one dye swap.

2010-05-06 | E-GEOD-18355 | biostudies-arrayexpress

Gene expression of Streptococcus mutans pknB mutant versus wild type strain

2010-03-05 | GSE18355 | GEO

Arabidopsis FTSH12 mutant terminomics

Project description:DEgradomic analysis of protein termini in Arabidopsis thaliana mutants expressing altered abundance of the chloroplast protease FTSH12.

2020-11-09 | PXD022423 | Pride

A Study Evaluating the Combination of Encorafenib and Cetuximab Versus Irinotecan/Cetuximab or Infusional 5-fluorouracil (5-FU)/Folinic Acid (FA)/Irinotecan (FOLFIRI)/Cetuximab in Chinese Patients With BRAF V600E Mutant Metastatic Colorectal Cancer.

Project description:Encorafenib is currently being developed (with or without binimetinib), in combination with cetuximab, for the treatment of adult patients with B-RAF proto-oncogene, serine/threonine kinase V600E mutant (BRAF V600E) metastatic colorectal cancer (mCRC), who have received prior systemic therapy.

| 2386568 | ecrin-mdr-crc

The yeast PP2A-CDC55 phosphatase regulates the transcriptional response to hyperosmolarity stress by regulating Msn2 and Msn4

Project description:The yeast PP2A-Cdc55 Serine/Threonine phosphatase regulates transcription under certain conditions. It is required for full activation of the environmental stress response mediated by the transcription factors Msn2 and Msn4. PP2A-Cdc55 contributes to sustained nuclear accumulation of Msn2 and Msn4 and extended chromatin recruitment under stress conditions such as hyperosmolarity stress. Transcript profiles of Msn2 and Msn4 double mutants are similar to cdc55 and the corresponding triple mutants. This argues for a Msn2/4 specific function of PP2A-Cdc55. Time course of 10 20 and 30 minutes hyperosmolarity treated yeast cells of wild type (W303), msn2msn4, cdc55, msn2msn5cdc55 genetic background.

2012-06-07 | E-GEOD-38565 | biostudies-arrayexpress

Metabolite profiling of Arabidopsis mutants of enzymes of lower glycolysis

Project description:We have previously shown that in Arabidopsis the three enzymes of lower glycolysis phosphoglycerate mutase (PGAM), enolase and pyruvate kinase form a complex which play an important role in co-sub-cellar localization the mitochondria to the chloroplast. Given that their metabolites of these mutants, the complementation of pgam mutant and overexpression of PGAM are still unclear, here, we present the gas chromatography mass spectrometry-based metabolomics data and plant growth phenotypes of them. Compared with wild type, both sugar and amino acid are significantly changed at mutants of phosphoglycerate mutase, enolase, pyruvate kinase. Overexpression the PGAM could decrease the content of 3PGA, sugar and several amino acids and increase the content of alanine and pyruvate. In addition, pgam mutant could not be fully complemented by nuclear sub-localized, the side-directed-mutated and the E.coli PGAM at plant phenotype and metabolites profiling, especially the content of 3PGA, serine and intermediates of TCA cycle. These data suggested that the low glycolysis complex play an important role at both mitochondria-chloroplast co-subcellar localization and the metabolites exchange.

2022-10-10 | MTBLS4066 | MetaboLights