Proteomics

Dataset Information

0

Alterations of human plasma proteome profile on adaptation to high-altitude hypobaric hypoxia


ABSTRACT: For individuals migrating to or residing permanently at high-altitude regions, environmental hypobaric hypoxia is a primary challenge which induces several physiological or pathological responses. It is well documented that human beings adapt to hypobaric hypoxia via some protective mechanisms, such as erythropoiesis and overproduction of hemoglobin, however little is known on the changes of plasma proteome profiles in accommodation to high-altitude hypobaric hypoxia. In the present study, we investigated differential plasma proteomes of high altitude natives and lowland normal controls by a TMT-based proteomic approach. A total of 818 proteins were identified, of which 137 were differentially altered. Bioinformatics (including GO, KEGG, protein-protein interactions, etc.) analysis revealed the dysregulated proteins were primarily involved in complement and coagulation cascades, anti-oxidative stress and glycolysis. Validations via magnetic Luminex® Assays and ELISA demonstrated that CCL18, C9, PF4, MPO and S100A9 notably up-regulated, and HRG and F11 down-regulated in high altitude natives compared with lowland controls, which were consistent with the proteomic results. Our findings highlight the roles of complement and coagulation cascades, anti-oxidative stress and glycolysis in acclimatization to hypobaric hypoxia and provide a foundation for developing potential diagnostic or/and therapeutic biomarkers for high altitude hypobaric hypoxia-induced diseases.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: xiaonan fu  

LAB HEAD: Zongkui Wang

PROVIDER: PXD012221 | Pride | 2019-03-27

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
71182TQ_1.raw Raw
71182TQ_10.raw Raw
71182TQ_11.raw Raw
71182TQ_12.raw Raw
71182TQ_13.raw Raw
Items per page:
1 - 5 of 20
altmetric image

Publications

Alterations of Human Plasma Proteome Profile on Adaptation to High-Altitude Hypobaric Hypoxia.

Du Xi X   Zhang Rong R   Ye Shengliang S   Liu Fengjuan F   Jiang Peng P   Yu Xiaochuan X   Xu Jin J   Ma Li L   Cao Haijun H   Shen Yuanzhen Y   Lin Fangzhao F   Wang Zongkui Z   Li Changqing C  

Journal of proteome research 20190403 5


For individuals migrating to or residing permanently in high-altitude regions, environmental hypobaric hypoxia is a primary challenge that induces several physiological or pathological responses. It is well documented that human beings adapt to hypobaric hypoxia via some protective mechanisms, such as erythropoiesis and overproduction of hemoglobin; however, little is known on the alterations of plasma proteome profiles in accommodation to high-altitude hypobaric hypoxia. In the present study, w  ...[more]

Similar Datasets

2013-12-18 | E-GEOD-52209 | biostudies-arrayexpress
2013-12-24 | E-GEOD-43327 | biostudies-arrayexpress
2021-11-25 | PXD017057 | Pride
2010-07-31 | E-GEOD-15879 | biostudies-arrayexpress
2012-12-12 | E-GEOD-33898 | biostudies-arrayexpress
2017-02-09 | GSE89486 | GEO
2022-02-22 | PXD001112 | Pride
2022-02-15 | PXD028070 | Pride
2020-11-06 | GSE160926 | GEO
2013-08-15 | E-GEOD-49920 | biostudies-arrayexpress