Proteomics

Dataset Information

0

Delineation of the olive pollen proteome: A focus on its allergen families


ABSTRACT: The delineation of the olive pollen proteome and its allergogram can improve the clinical management of patients with this pollinosis. We here integrated the recently described wild olive genomic data in a comprehensive proteomic approach to get the annotated olive (Olea europaea) pollen proteome and complete its complex allergogram. Olive pollen proteins were identified by LC-MS/MS using predicted protein sequences from its genome. GO annotation, KEGG Pathway analysis and identification of allergen families were performed by bioinformatics. Recombinant DNA, protein expression and purification, and immunological analyses were used to characterize putative allergens. A total of 1,907 proteins were identified. 60% of the proteins were predicted to possess catalytic activity and be involved in metabolic processes. 203 proteins belonging to 47 allergen families were found, with 37 non-previously described in olive pollen. Of four potential allergens produced in Escherichia coli, a peptidyl-prolyl cis-trans isomerase -cyclophilin-, masked in the protein extract by the major allergen Ole e 1, was found as a new olive pollen allergen (Ole e 15). 63% of the Ole e 15-sensitized patients were children and showed strong IgE recognition of the allergen. Ole e 15 shared high sequence identity with other plant, animal and fungal cyclophilins and a high IgE cross-reactivity with pollen, plant food and animal extracts. Taken together, the combination of available genomic data with proteomics permitted the profiling of the olive pollen proteome, revealing the spectrum of allergen families and cyclophilin as a new relevant allergen implicated in cross-reactivity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Olea Europaea (common Olive)

TISSUE(S): Pollen

SUBMITTER: Rodrigo Barderas  

LAB HEAD: Rodrigo Barderas

PROVIDER: PXD012279 | Pride | 2019-06-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Ole-Fraccioones-1-6-Allergome.msf Msf
Ole-Fraccioones-1-6-Oleaeuropea.msf Msf
Ole1.raw Raw
Ole2.raw Raw
Ole3.raw Raw
Items per page:
1 - 5 of 8
altmetric image

Publications

Delineation of the Olive Pollen Proteome and Its Allergenome Unmasks Cyclophilin as a Relevant Cross-Reactive Allergen.

San Segundo-Acosta Pablo P   Oeo-Santos Carmen C   Benedé Sara S   de Los Ríos Vivian V   Navas Ana A   Ruiz-Leon Berta B   Moreno Carmen C   Pastor-Vargas Carlos C   Jurado Aurora A   Villalba Mayte M   Barderas Rodrigo R  

Journal of proteome research 20190627 8


Olive pollen is a major allergenic source worldwide due to its extensive cultivation. We have combined available genomics data with a comprehensive proteomics approach to get the annotated olive tree (<i>Olea europaea</i> L.) pollen proteome and define its complex allergenome. A total of 1907 proteins were identified by LC-MS/MS using predicted protein sequences from its genome. Most proteins (60%) were predicted to possess catalytic activity and be involved in metabolic processes. In total, 203  ...[more]

Similar Datasets

2019-06-17 | PXD012334 | Pride
2019-06-17 | PXD012280 | Pride
2013-02-28 | E-GEOD-37157 | biostudies-arrayexpress
2012-01-03 | E-GEOD-20020 | biostudies-arrayexpress
2010-09-08 | E-MTAB-177 | biostudies-arrayexpress
2019-07-31 | GSE117803 | GEO
2023-02-21 | PXD039324 | Pride
2017-06-23 | GSE72324 | GEO
2009-06-16 | E-MAXD-32 | biostudies-arrayexpress
2014-12-30 | GSE54522 | GEO