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Proteome analysis of xylose metabolism in Rhodotorula toruloides during lipid production

ABSTRACT: Our study is the first investigation of proteome of oleaginous yeast Rhotodorula toruloides during conversion of xylose into lipids. We performed comparative proteomics of R. toruloides during cultivation of two carbon sources: glucose and xylose. Proteome analysis revealed significantly lower levels of ribosomal proteins and translation associated factors in xylose-grown cells as compared to glucose-grown cells. We showed that proteins involved in sugar transport, phospholipid and leucine biosynthesis as well as peroxisomal beta-oxidation and oxidative stress response were differentially regulated in response to carbon source.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rhodotorula Toruloides

TISSUE(S): Vegetative Cell (sensu Fungi)

SUBMITTER: Ievgeniia Tiukova

LAB HEAD: Jens Nielsen

PROVIDER: PXD012332 | Pride | 2019-08-08

REPOSITORIES: Pride

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			Action	DRS
	20170620_SLU_A1G.raw	Raw
	20170901_SLU_A10X.raw	Raw
	20170901_SLU_A2G.raw	Raw
	20170901_SLU_A2X.raw	Raw
	20170901_SLU_A5X.raw	Raw

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Publications

Proteome analysis of xylose metabolism in <i>Rhodotorula toruloides</i> during lipid production.

Tiukova Ievgeniia A IA Brandenburg Jule J Blomqvist Johanna J Sampels Sabine S Mikkelsen Nils N Skaugen Morten M Arntzen Magnus Ø MØ Nielsen Jens J Sandgren Mats M Kerkhoven Eduard J EJ

Biotechnology for biofuels 20190604

<h4>Background</h4><i>Rhodotorula toruloides</i> is a promising platform organism for production of lipids from lignocellulosic substrates. Little is known about the metabolic aspects of lipid production from the lignocellolosic sugar xylose by oleaginous yeasts in general and <i>R. toruloides</i> in particular. This study presents the first proteome analysis of the metabolism of <i>R. toruloides</i> during conversion of xylose to lipids.<h4>Results</h4><i>Rhodotorula toruloides</i> cultivated o ...[more]

PMID: 31171938

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Absence of di-auxie during simultaneous utilization of glucose and xylose by Sulfolobus acidocaldarius

Project description:Sulfolobus acidocaldarius has been previously reported to grow on a broad range of sugars, but there is limited information on the ability of the organism to metabolize multiple sugars simultaneously. We report here the ability of S. acidocaldarius to utilize glucose and xylose simultaneously without di-auxie effect. The organism utilized a mixture of 1 g/L glucose and 1 g/L xylose with a growth rate of 0.079 h-1 compared to 0.074 h-1 and 0.22 h-1 when the organism was grown on xylose 2 g/L and 2 g/L glucose respectively as sole carbon sources. An increase in xylose concentration to 2 and 4 g/L in a medium containing 1 g/L glucose resulted in a growth rate of 0.082 and 0.085 h-1. However, increasing glucose concentration by 2 and 4 g/L when xylose concentration was maintained at 1 g/L decreased the growth rate to 0.062 and 0.052 h-1 respectively. S. acidocaldarius appeared to be utilizing the sugars at a rate roughly proportional to their concentration in the medium, resulting in complete utilization of these sugars at same time. The organism did not show preference for either glucose or xylose when it was grown on both sugars. Similar results were obtained with a combination of glucose, arabinose and galactose. These observations strongly suggest that S. acidocaldarius does not regulate utilization of the sugars tested herein using carbon catabolite repression (CCR) commonly found in most bacteria. The mechanism by which the organism utilized a mixture of sugar is yet to be elucidated. However, we were able to identify genes encoding for the putative glucose ABC transporters; but the putative xylose transporter was not identified. A study of S. acidocaldarius grown in glucose, xylose, or the two carbon sources combined in mid-log. Analyzed on Nimblegen 4-plex S. acidocaldarius DSM 639 array weblink: http://microbesonline.org/cgi-bin/microarray/viewExp.cgi?expId=1723

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