Proteomics

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Thylakoid protein phosphorylation in Physcomitrella patens

ABSTRACT: Physcomitrella patens is a model plant representing the group of mosses, which diverged from vascular plant ancestors early after land colonization. To investigate the unique characteristic of the composition and post-translational regulation of Physcomitrella patens photosynthetic apparatus, we have separated the thylakoids protein complexes in their subunits composition by means of two-dimensional large-pore Blue-Native/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2D lpBN/SDS-PAGE), followed by protein in-gel tryptic digestion of single spots. To identify the kinase responsible for phosphorylation of the major thylakoid protein complex photosystem II, a mutant line lacking the ortholog of kinase STN8 in the model angiosperm Arabidopsis thaliana has been generated and the identified thylakoid protein phosphorylation pattern have been compared with the WT moss.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Physcomitrella Patens Subsp. Patens (moss)

TISSUE(S): Plant Cell, Protonema

SUBMITTER: Andrea Trotta  

LAB HEAD: Eva-Mari Aro

PROVIDER: PXD012501 | Pride | 2019-05-08

REPOSITORIES: pride

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Dataset's files

Source:
Action DRS
ATPase_comigrating_SC6.msf Msf
ATPase_comigrating_SC6.mzML Mzml
ATPase_comigrating_SC6.mzid.gz Mzid
ATPase_comigrating_SC6.pride.mgf.gz Mgf
ATPasecomigratingSC6_overloaded.raw Raw
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Publications

Thylakoid Protein Phosphorylation Dynamics in a Moss Mutant Lacking SERINE/THREONINE PROTEIN KINASE STN8.

Gerotto Caterina C   Trotta Andrea A   Bajwa Azfar Ali AA   Mancini Ilaria I   Morosinotto Tomas T   Aro Eva-Mari EM  

Plant physiology 20190506 3

In all eukaryotes, protein phosphorylation is a key regulatory mechanism in several cellular processes, including the acclimation of photosynthesis to environmental cues. Despite being a well-conserved regulatory mechanism in the chloroplasts of land plants, distinct differences in thylakoid protein phosphorylation patterns have emerged from studies on species of different phylogenetic groups. Here, we analyzed thylakoid protein phosphorylation in the moss <i>Physcomitrella patens</i>, assessing  ...[more]

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