Proteomics

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Specific inhibition of splicing factor activity by decoy RNA oligonucleotides

ABSTRACT: Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of a RNA motif, which is recognized by a single SF. Here we show that decoy oligonucleotides targeting splicing factors RBFOX1/2, SRSF1 and PTBP1, can specifically bind to their respective SFs and inhibit their splicing and biological activities both in vitro and in vivo. These decoy oligonucleotides present a novel approach to specifically downregulate SF activity and have the potential to treat diseases where SFs are up-regulated, such as cancer.

OTHER RELATED OMICS DATASETS IN: GSE126503

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Tamar Geiger  

LAB HEAD: Tamar Geiger

PROVIDER: PXD012564 | Pride | 2019-01-31

REPOSITORIES: Pride

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Action DRS
Animal_20151223_GB_Collab_Polina_Fox_1.raw Raw
Animal_20151223_GB_Collab_Polina_Fox_2.raw Raw
Animal_20151223_GB_Collab_Polina_Fox_3.raw Raw
Animal_20151223_GB_Collab_Polina_Inhibitor_1.raw Raw
Animal_20151223_GB_Collab_Polina_Inhibitor_2.raw Raw
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Alternative splicing, a fundamental step in gene expression, is deregulated in many diseases. Splicing factors (SFs), which regulate this process, are up- or down regulated or mutated in several diseases including cancer. To date, there are no inhibitors that directly inhibit the activity of SFs. We designed decoy oligonucleotides, composed of several repeats of a RNA motif, which is recognized by a single SF. Here we show that decoy oligonucleotides targeting splicing factors RBFOX1/2, SRSF1 an  ...[more]

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