Proteomics

Dataset Information

0

Rapid Assay of Individual Microbiome responses to drugs data part2

ABSTRACT: We developed an approach named Rapid Assay of Individual Microbiome (RapidAIM) to screen xenobiotics against individual microbiomes. To evaluate technical reproducibility, we tested 43 compounds against an individual's microbiome. The individual microbiome is cultured in triplicates in 96-well plates for 24 hours and the samples are then analyzed using a metaproteomics-based analytical approach to gain functional insight into the individual microbiomes responses following drug treatments.The tested compounds significantly affected overall microbiome abundance, microbiome composition and functional pathways at multiple taxonomic levels. The microbiome responses to berberine, metformin, diclofenac, fructooligosaccharide and most antibiotics were consistent among most individual microbiomes. Interestingly, most of our tested NSAIDs, statins, and histamine-2 blockers induced individually distinct responses. Our workflow offers an effective solution to systematically study the effects of many different compounds on individual microbiomes.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: Leyuan Li  

LAB HEAD: Daniel Figeys

PROVIDER: PXD012725 | Pride | 2020-05-27

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
Leyuan_20170610_CpdPreTest_P1A1.raw Raw
Leyuan_20170610_CpdPreTest_P1A10.raw Raw
Leyuan_20170610_CpdPreTest_P1A11.raw Raw
Leyuan_20170610_CpdPreTest_P1A12.raw Raw
Leyuan_20170610_CpdPreTest_P1A2.raw Raw
Items per page:
1 - 5 of 185
altmetric image

Publications

RapidAIM: a culture- and metaproteomics-based Rapid Assay of Individual Microbiome responses to drugs.

Li Leyuan L   Ning Zhibin Z   Zhang Xu X   Mayne Janice J   Cheng Kai K   Stintzi Alain A   Figeys Daniel D  

Microbiome 20200311 1

<h4>Background</h4>Human-targeted drugs may exert off-target effects or can be repurposed to modulate the gut microbiota. However, our understanding of such effects is limited due to a lack of rapid and scalable assay to comprehensively assess microbiome responses to drugs. Drugs and other compounds can drastically change the overall abundance, taxonomic composition, and functions of a gut microbiome.<h4>Results</h4>Here, we developed an approach to screen compounds against individual microbiome  ...[more]

Similar Datasets

Proteomics Rapid Assay of Individual Microbiome responses to drugs part1
2020-05-26 | PXD012724 | Pride
Proteomics Effect of berberine analogues on functional profiles of human gut microbiomes
2020-07-21 | PXD015934 | Pride
Transcriptomics HuMiChip analysis of 86 samples from different body sites
2014-02-04 | E-GEOD-54290 | biostudies-arrayexpress
Proteomics Metaproteomic responses of in vitro gut microbiomes to resistant starches
2021-09-08 | PXD017907 | Pride
Transcriptomics RNA-seq of C. elegans reared on experimental microbiome (EM) and EM cell-free media against OP50-fed controls
2022-06-23 | E-MTAB-10693 | biostudies-arrayexpress
Proteomics Functional effects of sweeteners on ex vivo human gut microbiome
2022-06-23 | PXD030458 | Pride
Proteomics Quantitative metaproteomics and activity-based probe enrichment on fecal samples from a mouse model of inflammatory bowel disease
2017-01-16 | PXD005667 | Pride
Genomics Effect of morphine treatment on microbiome in the context of microbial transplant.
2016-06-01 | E-MTAB-3723 | biostudies-arrayexpress
Other HuMiChip analysis of 86 samples from different body sites
2014-02-04 | GSE54290 | GEO
Proteomics Fecal Transplant from Roux-Y-Gastric-Bypass rat model into a diet induced obesity rat model
2022-08-12 | PXD026606 | Pride