Dataset Information

Functional effects of sweeteners on ex vivo human gut microbiome

ABSTRACT: We systematically investigated the responses of five human gut microbiomes to 21 common sweeteners, using an approach combining high-throughput ex vivo microbiome culturing and metaproteomics to quantify functional changes in different taxa. Hierarchical clustering based on metaproteomic responses of individual microbiomes resulted in two clusters. The first cluster was composed of non-caloric artificial sweeteners (NAS) and two sugar alcohols with shorter carbon backbones (4-5 carbon atoms), and the second cluster was composed of sugar alcohols with longer carbon backbones. The metaproteomic functional responses of the second cluster were similar to the prebiotic fructooligosaccharides and kestose, indicating that these sugar alcohol-type sweeteners have potential prebiotic functions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Human Gut Metagenome

SUBMITTER: Leyuan Li

LAB HEAD: Daniel Figeys

PROVIDER: PXD030458 | Pride | 2022-06-23

REPOSITORIES: pride

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			Action	DRS
	Search.zip	Other
	Wenju_202003_Sweeteners_QC_02.raw	Raw
	Wenju_202003_Sweeteners_QC_03.raw	Raw
	Wenju_202003_Sweeteners_QC_04.raw	Raw
	Wenju_202003_Sweeteners_QC_05.raw	Raw

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Publications

Comprehensive Assessment of Functional Effects of Commonly Used Sugar Substitute Sweeteners on <i>Ex Vivo</i> Human Gut Microbiome.

Sun Zhongzhi Z Wang Wenju W Li Leyuan L Zhang Xu X Ning Zhibin Z Mayne Janice J Walker Krystal K Stintzi Alain A Figeys Daniel D

Microbiology spectrum 20220613 4

The composition and function of the human gut microbiome are often associated with health and disease status. Sugar substitute sweeteners are widely used food additives, although many studies using animal models have linked sweetener consumption to gut microbial changes and health issues. Whether sugar substitute sweeteners directly change the human gut microbiome functionality remains largely unknown. In this study, we systematically investigated the responses of five human gut microbiomes to 2 ...[more]

PMID: 35695565

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