Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Barnacle cyprid cement gland LC-MS/MS

ABSTRACT: Proteins were extracted from the cement glands of the cypird barnacle Megabalanus volcano. And thenproteins were subjected to in-solution digestion and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Megabalanus Volcano

TISSUE(S): Cement Gland

SUBMITTER: Guoyong Yan

LAB HEAD: Lisheng He

PROVIDER: PXD012779 | Pride | 2021-09-08

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	F001666_cg.csv	Csv
	cg_trypsin_151106182940.mgf	Mgf
	cg_trypsin_151106182940.raw	Raw

Items per page:

1 - 3 of 3

Publications

Insights into the Synthesis, Secretion and Curing of Barnacle Cyprid Adhesive via Transcriptomic and Proteomic Analyses of the Cement Gland.

Yan Guoyong G Sun Jin J Wang Zishuai Z Qian Pei-Yuan PY He Lisheng L

Marine drugs 20200331 4

Barnacles represent one of the model organisms used for antifouling research, however, knowledge regarding the molecular mechanisms underlying barnacle cyprid cementation is relatively scarce. Here, RNA-seq was used to obtain the transcriptomes of the cement glands where adhesive is generated and the remaining carcasses of <i>Megabalanus</i> <i>volcano</i> cyprids. Comparative transcriptomic analysis identified 9060 differentially expressed genes, with 4383 upregulated in the cement glands. Four ...[more]

PMID: 32244485

Similar Datasets

Amphibalanus amphitrite transcriptomics and proteomics - cement proteins

Project description:A complete understanding of barnacle adhesion remains elusive as the process occurs within and beneath the confines of a rigid calcified shell. Barnacle cement is mainly proteinaceous and several individual proteins have been identified in the hardened cement at the barnacle-substrate interface.We report the discovery of a novel 114kD cement protein, which is identified in material secreted onto various surfaces by adult barnacles and with the encoding gene highly expressed in the sub-mantle tissue.

2015-10-20 | PXD001982 | Pride

Apple snail perivitelline fluid LC-MS/MS

Project description:Proteins were extracted from the perivitelline fluid of the apple snail Pomacea maculata eggs. And then proteins were seperated on SDS-PAGE gel and analyzed by LTQ-Orbitrap Elite coupled to an Easy-nLC.

2017-09-25 | PXD006718 | Pride

Comparative analysis of stalked and acorn barnacle adhesive proteomesv

Project description:Barnacles interest the scientific community for multiple reasons: their unique evolutionary trajectory, vast diversity, and economic impact as a harvested food source and also as one of the most prolific macroscopic hard biofouling organisms. A common, yet novel, trait among barnacles is adhesion, which has enabled a sessile adult existence and global colonization of the oceans. Barnacle adhesive is primarily composed of proteins, but knowledge of how the adhesive proteome varies across the tree of life is unknown due to a lack of genomic information. Here, we supplement previous mass spectrometry analyses of barnacle adhesive with recently sequenced genomes to compare the adhesive proteomes of Pollicipes pollicipes (Pedunculata) and Amphibalanus amphitrite (Sessilia). Although both species contain the same broad protein categories, we detail differences that exist between these species. The barnacle-unique cement proteins show the greatest difference between species, although these differences are diminished when amino acid composition and glycosylation potential are considered. By performing an in-depth comparison of the adhesive proteomes of these distantly related barnacle species, we show their similarities and provide a roadmap for future studies examining sequence specific differences to identify the proteins responsible for functional differences across the barnacle tree of life.

2022-02-17 | PXD026105 | Pride

Project description:cement gland transcriptome of barnacle Megabalanus volcano cyprid

| PRJNA431879 | ENA

Sequence basis of Barnacle Cement Nanostructure is Defined by Proteins with Silk Homology

Project description:Study to identify proteins present at the barnacle substrate interface. These proteins are candidates for adhesion proteins.

2016-11-23 | PXD004293 | Pride

LncRNA NONMMUT104330.1-miR-709-DDX58 axis mediates enoxaparin sodium bone cement to regulate M2 polarization of macrophages and play an anti-inflammatory immunomodulatory role

Project description:PMMA bone cement is biologically inert and exhibits poor biological activity. Following implantation, it generates bone cement particles, which can easily induce tissue inflammation around the implant . Compared with PMMA bone cement, ES-PMMA bone cement had anti-inflammatory effect by inducing M2 polarization of macrophages. RNA sequencing was used to explore the underlying molecular mechanisms.

2024-10-24 | GSE279658 | GEO

Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.

Project description:Ixodes scapularis and other hard ticks are long-term blood feeders. This feeding behavior is facilitated by a tick secreted bio adhesive (tick cement) that attaches tick mouthparts to skin tissue and prevents the host from dislodging the tick from its feeding site. Understanding tick cement formation is highly sought after as its disruption will prevent tick feeding. This study describes proteins that form the inner core layer of I. scapularis tick cement as disrupting these will stop formation of the outer cortical layer. As the inner core cement layer completes forming by 24 h of attachment, we used laser capture to isolate cement from cryosections of 6 h and 24 h tick attachment sites and to distinguish between early and late inner core cement proteins. LC-MS/MS analysis identified 138 tick cement proteins (TCPs) of which 37 and 35 were unique in cement of 6 and 24 h respectively, and 66 shared proteins. The 138 TCPs are classified in 14 functional categories: cuticular protein (16%), tick specific proteins of unknown function, cytoskeletal proteins, and enzymes (at 13% each), enzymes (10%), antioxidant, glycine rich, scaffolding, heat shock, histone, histamine binding, proteases and protease inhibitors, and miscellaneous (at 3-6% each). Gene ontology analysis confirm that TCPs are enriched for bio adhesive properties. Our data offer insights into yet unknown tick cement bonding patterns and set the foundation for understanding the molecular basis of I. scapularis tick cement formation.

2023-03-11 | PXD038685 | Pride

Bacteria isolated from the cement

Project description:Bacteria isolated from the cement capable of forming calcium carbonate crystals

| PRJEB32639 | ENA

The mode of action of antifouling compound albofungin in inhibiting barnacle settlement

Project description:In the present study, proteomic analyses were used to investigate changes in the transcriptome and proteome of a major fouling barnacle Amphibalanus amphitrite cyprids in response to albofungin treatment.

2023-02-21 | PXD039255 | Pride

Two goose barnacles species (goose neck barnacle & pelagic gooseneck barnacle)

Project description:Cement gland transcriptomes of 2 goose barnacles species (Pollicipes pollicipes and Lepas anatifera)

| PRJNA437397 | ENA