Proteomics

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Analysis of bacterial Type VI Secretion Systems Stoichiometry by Targeted Proteomics


ABSTRACT: The bacterial Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria, including human pathogens. T6SS is evolutionarily related to the contractile phage tails and assembles as a cell envelope attached organelle. The assembly progresses from formation of a membrane complex to assembly of a baseplate followed by copolymerization of a sheath-tube. Rapid sheath contraction propels the rigid inner tube with associated effectors into target cells. To understand the assembly and stoichiometry of this nanomachine, we applied targeted proteomics to determine the protein abundances of the key T6SS components in three model bacteria. The overall stoichiometry of the components is conserved across species and it agrees with the expected composition of the nanomachine. However, there are also species-specific variations of certain components, which may explain the observed differences in the respective dynamics of T6SS. Furthermore, changes in the protein abundance during different growth conditions point to possible mechanisms of regulation of T6SS dynamics. The combination of live-cell imaging and mass-spectrometry analysis suggests that a baseplate component TssE of V. cholerae might undergo an active proteolysis, which together with an effector protein VasX could be involved in regulation of baseplate dynamics and thus T6SS activity.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Vibrio Sp. 57b112y11

TISSUE(S): Cell Culture

SUBMITTER: Alexander Schmidt  

LAB HEAD: Alexander Schmidt

PROVIDER: PXD012832 | Pride | 2019-06-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
001_Celspik3_mix1r_B17.raw Raw
001_Celspik3_mix2_B17.raw Raw
001_Celspik4_B17.raw Raw
001_Celspik4_mix2_B17.raw Raw
001_Celspik5-mix2_B17.raw Raw
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Publications

Abundance of bacterial Type VI secretion system components measured by targeted proteomics.

Lin Lin L   Lezan Emmanuelle E   Schmidt Alexander A   Basler Marek M  

Nature communications 20190613 1


The Type VI secretion system (T6SS) is important for bacterial competition as well as virulence in many Gram-negative bacteria and its dynamics and regulation varies significantly between species. To gain insights into the mechanisms regulating T6SS assembly, we apply targeted proteomics to determine the abundance of the key T6SS components in Vibrio cholerae, Pseudomonas aeruginosa and Acinetobacter baylyi. We show that while there are species specific exceptions, the abundance of most componen  ...[more]

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