Proteomics

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Approaching absolute quantification from chemostats cultures of Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS 6556 and Yarrowia lipolytica W29


ABSTRACT: Protein extracts of three yeast strains (Saccharomyces cerevisiae CEN.PK113-7D, Kluyveromyces marxianus CBS6556 and Yarrowia lipolytica W29) cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions for each yeast strain were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: - Standard condition : 30°C, pH 5.5 - High temperature: 36°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress : 30°C, pH 5.5, 1M KCl The conditions for Kluyveromyces marxianus CBS6556 are: - Standard condition : 30°C, pH 5.5 - High temperature: 40°C, pH 5.5 - Low pH: 30°C, pH 3.5 - Osmotic stress: 30°C, pH 5.5, 0.6 M KCl The conditions for Yarrowia lipolytica W29 are: - Standard condition: 28°C, pH 5.5 - High temperature: 32°C, pH 5.5 - Low pH: 28°C, pH 3.5 This study is part of the OMICS data generation WP of CHASSY project (European Union’s Horizon 2020 grant agreement No 720824).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Yarrowia Lipolytica (candida Lipolytica) Kluyveromyces Marxianus Dmku3-1042 Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Aaron Millan Oropeza  

LAB HEAD: Véronique Monnet

PROVIDER: PXD012836 | Pride | 2022-02-15

REPOSITORIES: Pride

Dataset's files

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Action DRS
20180802_04_SC_Bulk_A.mzXML Mzxml
20180802_04_SC_Bulk_A.xml Xml
20180802_06_SC_T2A_A.mzXML Mzxml
20180802_06_SC_T2A_A.xml Xml
20180802_08_SC_T3A_A.mzXML Mzxml
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Publications

Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance.

Millán-Oropeza Aarón A   Blein-Nicolas Mélisande M   Monnet Véronique V   Zivy Michel M   Henry Céline C  

Proteomes 20220107 1


In proteomics, it is essential to quantify proteins in absolute terms if we wish to compare results among studies and integrate high-throughput biological data into genome-scale metabolic models. While labeling target peptides with stable isotopes allow protein abundance to be accurately quantified, the utility of this technique is constrained by the low number of quantifiable proteins that it yields. Recently, label-free shotgun proteomics has become the "gold standard" for carrying out global  ...[more]

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