Proteomics

Dataset Information

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Approaching absolute quantification using label-free approaches and external proteins standards in yeast cultures


ABSTRACT: Protein extracts of Saccharomyces cerevisiae CEN.PK113-7D cultivated in chemostats under different conditions. Representative samples containing aliquots of all conditions were spiked with UPS2 standard (Sigma) to estimate absolute values in fmol. The conditions for Saccharomyces cerevisiae CEN.PK113-7D are: T2- Standard condition : 30°C, pH 5.5 T3- High temperature: 36°C, pH 5.5 T4- Low pH: 30°C, pH 3.5 T5- Osmotic stress : 30°C, pH 5.5, 1M KCl T6- Anaerobic condition Furthermore, representative samples pooling aliquots of each condition are indicated as "bulk" samples. These samples were spiked with UPS proteins. A validation step was carried out by spiking 4 external proteins at known concentrations within the yeast and UPS proteins mixture

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Aaron Millan Oropeza  

LAB HEAD: Celine Henry

PROVIDER: PXD014765 | Pride | 2022-02-15

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
20180802_04_SC_Bulk_A.mzXML Mzxml
20180802_04_SC_Bulk_A.xml Xml
20180802_06_SC_T2A_A.mzXML Mzxml
20180802_06_SC_T2A_A.xml Xml
20180802_08_SC_T3A_A.mzXML Mzxml
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Publications

Comparison of Different Label-Free Techniques for the Semi-Absolute Quantification of Protein Abundance.

Millán-Oropeza Aarón A   Blein-Nicolas Mélisande M   Monnet Véronique V   Zivy Michel M   Henry Céline C  

Proteomes 20220107 1


In proteomics, it is essential to quantify proteins in absolute terms if we wish to compare results among studies and integrate high-throughput biological data into genome-scale metabolic models. While labeling target peptides with stable isotopes allow protein abundance to be accurately quantified, the utility of this technique is constrained by the low number of quantifiable proteins that it yields. Recently, label-free shotgun proteomics has become the "gold standard" for carrying out global  ...[more]

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