Dataset Information

Endo-Lysosomal and Ubiquitin CSF Biomarker Discovery by solid-phase extraction and LC-MS/MS

ABSTRACT: Dysfunctional proteostasis is increasingly implicated in neurodegenerative diseases which is exemplified by an accumulation of protein aggregates in the brain in e.g. Alzheimer's and Parkinson's disease. In addition, in Alzheimer's disease there is an accumulation of autophagic vesicles within neurites. Susceptibility in Parkinson's disease has convincingly been connected with genetic mutations associated with lysosomal storage disorders. Proteostasis is maintained in the cell by the endo-lysosomal system, autophagy and the ubiquitin-proteasome system. Identifying biomarkers reflecting proteostasis might increase our understanding of neurodegenerative disorders and serve as tools to monitor the effects of new treatment strategies. To date no treatment has proven effective, neither in Alzheimer’s disease nor Parkinson’s disease. The aim of this project was to identify proteins involved in the endo-lysosomal system, autophagy and the ubiquitin-proteasome system in CSF. This information will be used to select and target relevant proteins for quantitative analysis using e.g. PRM or SRM to evaluate these proteins as potential biomarkers in neurodegenerative diseases.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Simon Sjödin

LAB HEAD: Henrik Zetterberg

PROVIDER: PXD012851 | Pride | 2019-09-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	150303_1_01FA_MoInt_HCDOT_03-1.msf	Msf
	150303_1_01FA_MoInt_HCDOT_03-1.pep.xml	Pepxml
	150303_1_01FA_MoInt_HCDOT_03.raw	Raw
	150303_2_01FA_MoInt_HCDOT_04-1.msf	Msf
	150303_2_01FA_MoInt_HCDOT_04-1.pep.xml	Pepxml

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Publications

Endo-lysosomal proteins and ubiquitin CSF concentrations in Alzheimer's and Parkinson's disease.

Sjödin Simon S Brinkmalm Gunnar G Öhrfelt Annika A Parnetti Lucilla L Paciotti Silvia S Hansson Oskar O Hardy John J Blennow Kaj K Zetterberg Henrik H Brinkmalm Ann A

Alzheimer's research & therapy 20190914 1

<h4>Background</h4>Increasing evidence implicates dysfunctional proteostasis and the involvement of the autophagic and endo-lysosomal system and the ubiquitin-proteasome system in neurodegenerative diseases. In Alzheimer's disease (AD), there is an accumulation of autophagic vacuoles within the neurons. In Parkinson's disease (PD), susceptibility has been linked to genes encoding proteins involved in autophagy and lysosomal function, as well as mutations causing lysosomal disorders. Furthermore, ...[more]

PMID: 31521194

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