Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

40 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from endothelial cells sorted from breast cancer cells MDA-MB231 as compared with normal endothelial cells

ABSTRACT: We studied the crosstalk between tumor and endothelial cells to explore the role of tumor microenvironment on cancer growth and progression. As part of our investigation, we showed that contact-dependent interaction of endothelial cells with breast tumor cells triggered the differential expression of a large number of genes in endothelium. Endothelial and MDA-MB231 cells were co-cultured together under serum- cytokine-free environment for 5 days followed by sorting endothelial cells and extracting RNA for microarray analysis. All conditions were made in triplicate. Endothelial cells were obtained using the methods described in Seandel M. & Butler J.M., 2008. WCMC-Q Genomics Core

ORGANISM(S): Homo sapiens

SUBMITTER: Najeeb Halabi

PROVIDER: E-GEOD-48786 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from endothelial cells sorted from breast cancer cells MDA-MB231 as compared with normal endothelial cells

Project description:We studied the crosstalk between tumor and endothelial cells to explore the role of tumor microenvironment on cancer growth and progression. As part of our investigation, we showed that contact-dependent interaction of endothelial cells with breast tumor cells triggered the differential expression of a large number of genes in endothelium.

2013-12-31 | GSE48786 | GEO

GNPS - Multi-omics analysis of endothelial cells reveals the metabolic diversity that underlies endothelial cell functions

Project description:Intra- and extracellular metabolomics dataset of human dermal blood endothelial cells (HDBECs), human umbilical vein endothelial cells (HUVECs), human dermal lymphatic endothelial cells (HDLECs) and intestinal lymphatic endothelial cells (iLECs) in proliferation and quiescence.

2024-11-13 | MSV000096398 | GNPS

CD34 marks angiogenic tip cells in human vascular endothelial cell cultures.

Project description:The functional shift of quiescent endothelial cells into tip cells that migrate and stalk cells that proliferate is a key event during sprouting angiogenesis. We previously showed that the sialomucin CD34 is expressed in a small subset of cultured endothelial cells and that these cells extend filopodia: a hallmark of tip cells in vivo. In the present study, we characterized endothelial cells expressing CD34 in endothelial monolayers in vitro. We found that CD34-positive human umbilical vein endothelial cells show low proliferation activity and increased mRNA expression of all known tip cell markers, as compared to CD34- negative cells. Genome-wide mRNA profiling analysis of CD34-positive endothelial cells demonstrated enrichment for biological functions related to angiogenesis and migration, whereas CD34-negative cells were enriched for functions related to proliferation. In addition, we found an increase or decrease of CD34-positive cells in vitro upon exposure to stimuli that enhance or limit the number of tip cells in vivo, respectively. Our findings suggest cells with virtually all known properties of tip cells are present in vascular endothelial cell cultures and that they can be isolated based on expression of CD34. This novel strategy may open alternative avenues for future studies of molecular processes and functions in tip cells in angiogenesis. Four HUVEC donors were FACS-sorted by CD34 and divided in CD34-positive and CD34-negative fractions.

2012-01-05 | E-GEOD-34850 | biostudies-arrayexpress

Oxidoreductase transcript data from human umbilical vein endothelial cells

Project description:Oxidoreductase enzymes are critical to redox regulation of intracellular proteins within human cells. We used microarrays to identify which oxidreducatse genes are expressed in unstimulated human umbilical vein endothelial cells. Human umbilical vein endothelial cells were grown under optimal conditions and then RNA extracted and hybridized on Affymetrix microarrays.

2010-06-16 | E-GEOD-22166 | biostudies-arrayexpress

Quiescent vs. Activated Endothelial Cell-Derived Extracellular Vesicles

Project description:Proteomics was performed on extracellular vesicles derived from in vitro cultures of activated and quiescent human endothelial cells.

2024-01-04 | PXD038914 | Pride

Transcription profiling time series of human endothelial cells invading into a three-dimensional collagen matrix

Project description:Gene expression profiling studies were performed at various times using a defined in vitro endothelial cell invasion assay system. Total RNA from invading cells was isolated at 0, 6, 12 and 18 hours and subjected to microarray analyses.

2008-10-01 | E-MEXP-1800 | biostudies-arrayexpress

Endothelial Response to Increased Shear Stress Frequency

Project description:Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress frequency (2 Hz) was then applied. The transcriptomics studies using microarray identified genes that were sensitive to the elevated shear frequency. keywords: adaptation, shear stress, frequency, microarray, gene expression Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress magnitude (2 Hz) was then applied. Gene expression at multiple time points was measured using microarray.

2011-12-01 | E-GEOD-26513 | biostudies-arrayexpress

The Adaptive Response of Endothelial Cells to Increased Shear Stress

Project description:In this study, we characterized the adaptive response of arterial endothelial cells to acute increases in shear stress magnitude in well-defined in vitro settings. Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress magnitude (30 ± 15 dynes/cm2) was then applied. The transcriptomics studies using microarray identified genes that were sensitive to the elevated shear magnitude. A significant number of the identified genes in our study are previously unknown as sensitive to shear stress. Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress magnitude (30 ± 15 dynes/cm2) was then applied. Gene expression at multiple time points was measured using microarray.

2011-12-01 | E-GEOD-26505 | biostudies-arrayexpress

Effect of Alloantibody and Complement on Endothelial Cells

Project description:Examine the possible pro-inflammatory gene effects of alloantibody and complement on endothelial cells Microarray 3 Experiment performed three times on three separate microarrays using three separate HUVEC and PRA donors. In each experiment, there are four groups of HUVEC with three replicates per group treated for 4 hours in gelatin veronal buffer containing either complement-inactivated PRA serum, vehicle (gelatin veronal buffer), PRA serum, or non-PRA serum

2013-08-28 | E-GEOD-50112 | biostudies-arrayexpress

Transcriptional effects of TNF-Î± and TGF-Î²1 on human umbilical vein endothelial cells

Project description:Transcriptional profiling of human umbilical vein endothelial cells following stimulation with tumour necrosis factor alpha and transforming growth factor beta singly or combined for 8 hr All stimulations were for 8 hr - TNF-Î± vs no cytokine; TGF-Î²1 vs no cytokine; TNF-Î± & TGF-Î²1 vs TNF-Î± alone; TNF-Î± & TGF-Î²1 vs TGF-Î²1 alone

2012-10-31 | E-GEOD-33416 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data