Dataset Information

Detecting single amino acids and small peptides by combining isobaric tags and peptidomics

ABSTRACT: Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identification approach. While there is still room for improvement, our simple test indicates that a limited amount of extra work when setting up the mass spectrometry experiment could potentially lead to a wealth of additional information.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebrospinal Fluid

SUBMITTER: Ragnhild Reehorst Lereim

LAB HEAD: Harald Barsnes

PROVIDER: PXD012872 | Pride | 2019-06-26

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20171024_EndoCSF_TMT_Rest_Charge1.raw	Raw
	Identifications.tsv	Tabular

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Publications

Detecting single amino acids and small peptides by combining isobaric tags and peptidomics.

Burger Bram B Lereim Ragnhild Reehorst RR Berven Frode S FS Barsnes Harald H

European journal of mass spectrometry (Chichester, England) 20190612 6

Single amino acids and small endogenous peptides play important roles in maintaining a properly functioning organism. These molecules are however currently only routinely identified in targeted approaches. In a small proof-of-concept mass spectrometry experiment, we found that by combining isobaric tags and peptidomics, and by targeting singly charged molecules, we were able to identify a significant amount of single amino acids and small endogenous peptides using a basic mass-based identificati ...[more]

PMID: 31189351

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