Dataset Information

Snap-heated freeze-free preservation and processing of the urine proteome

ABSTRACT: New method for urine proteome preservation and processing based on thermal stabilization and FASP digestion. Four urine proteomes were preserved during 48 h and 6 months using (i) the classic freeze preservation at -20 °C, (ii) snap-heated freeze-free preservation at laboratory temperature (20 °C) and (iii) snap-heated preservation at -60 °C.

INSTRUMENT(S): impact HD

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine

SUBMITTER: Hugo M. Santos

LAB HEAD: Hugo Miguel Baptista Carreira dos Santos

PROVIDER: PXD012880 | Pride | 2020-01-10

REPOSITORIES: Pride

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Snap-heated freeze-free preservation and processing of the urine proteome using the combination of stabilizor-based technology and filter aided sample preparation.

Carvalho Luís B LB Capelo-Martínez José Luis JL Lodeiro Carlos C Wiśniewski Jacek R JR Santos Hugo M HM

Analytica chimica acta 20190524

We present a method to preserve and process urine proteins for proteomic analysis in a filter aided sample preparation (FASP) format. The method combines concentration of urine proteins in ultrafiltration devices, their thermal stabilization, allowing long term storage of the samples, and filter aided sample preparation. Proteomes of four different urines were preserved during 48 h and 6 months using (i) the classic freeze preservation at -20 °C, (ii) snap-heated freeze-free preservation at labo ...[more]

PMID: 31203967

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			Action	DRS
	A1_T0_U2_1260.baf	Other
	A1_T0_U3_1447.baf	Other
	A1_T0_U4_1473.baf	Other
	A1_T0_U5_1499.baf	Other
	A1_T2_U2_1435.baf	Other