Proteomics

Dataset Information

0

Comparative proteomics of clinically relevant proteins in modern and traditional bread wheat cultivars


ABSTRACT: Bread wheat (Triticum aestivum L.) is one of the most valuable cereal crops for the human consumption. Its grain storage proteins greatly impact bread quality, though may cause food intolerances or allergies in susceptible individuals. Consequently, the investigation of a proteome polymorphism among wheat varieties is important to spot the genotypes, which would be promising donors for the breeding of hypoallergenic cereals. Herein, we discovered diversity of grain proteins in three Ukrainian wheat cultivars: ‘Sotnytsia’, ‘Panna’ (both modern selection) and ‘Ukrainka’ (old landrace). Firstly, proteins were isolated with a SDS-containing buffer that allowed extraction of various groups of storage proteins (glutenins, gliadins, globulins and albumins). Secondly, the proteome was profiled by the two-dimensional gel electrophoresis, revealing 810 clearly-separated gel spots. Software-assisted analysis of gel images, showed 66 differentially abundant proteins. Using multi-enzymatic digestion, followed by the tandem mass spectrometry, we identified 49 differentially accumulated proteins. Parallel ultrahigh-performance liquid chromatography profiling and direct mass spectrometry quantification complemented the results. With this approach we quantified 127 proteins, 12 being differentially abundant. Principal component analysis confirmed genotype as a major source of variation in both cases. Non-gluten fraction was the most diverse among investigated bread wheat cultivars. Information from public databases of clinically relevant plant proteins highlighted variable groups of wheat allergens/toxins. Data suggested that one of the modern cultivars contained less health affecting proteins in grain. Finally, we proposed set of genetic landmarks for the development of DNA marker system, which will enable fast and efficient assessment of medical safety of multiple wheat genotypes to facilitate breeding programs.

INSTRUMENT(S): Q-Tof Premier, SYNAPT G2-Si

ORGANISM(S): Triticum Aestivum (wheat)

TISSUE(S): Plant Cell, Seed

DISEASE(S): Celiac Disease,Food Allergy,Respiratory Allergy

SUBMITTER: Maksym Danchenko  

LAB HEAD: Andrej Kovac

PROVIDER: PXD012940 | Pride | 2020-05-20

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
1025.mzid.gz Mzid
1025.zip Other
1051.mzid.gz Mzid
1051.zip Other
1066.mzid.gz Mzid
Items per page:
1 - 5 of 200
altmetric image

Publications

Comprehensive Comparison of Clinically Relevant Grain Proteins in Modern and Traditional Bread Wheat Cultivars.

Lakhneko Olha O   Danchenko Maksym M   Morgun Bogdan B   Kováč Andrej A   Majerová Petra P   Škultéty Ľudovit Ľ  

International journal of molecular sciences 20200513 10


Bread wheat (<i>Triticum aestivum</i> L.) is one of the most valuable cereal crops for human consumption. Its grain storage proteins define bread quality, though they may cause food intolerances or allergies in susceptible individuals. Herein, we discovered a diversity of grain proteins in three Ukrainian wheat cultivars: Sotnytsia, Panna (both modern selection), and Ukrainka (landrace). Firstly, proteins were isolated with a detergent-containing buffer that allowed extraction of various groups  ...[more]

Similar Datasets

2023-07-28 | PXD040279 | Pride
2014-07-18 | E-MTAB-2137 | biostudies-arrayexpress
2021-05-30 | PXD021706 | Pride
2017-10-30 | PXD002805 | Pride
2015-04-24 | E-MTAB-3103 | biostudies-arrayexpress
2021-10-21 | MSV000088253 | MassIVE
2005-10-05 | E-MEXP-309 | biostudies-arrayexpress
2015-12-02 | GSE75561 | GEO
2022-10-01 | E-MTAB-9006 | biostudies-arrayexpress
2020-11-24 | GSE162027 | GEO