Dataset Information

Identification of DNA-damage sensing factors in D.melanogaster

ABSTRACT: In order to identify protein involved in sensing DNA double strand breaks and recruiting the repair machinry, we performed in vitro assembly of chromatin on small circular and linear DNA templates, respectively. Proteins involved in chromatin assembly and sensing of free DNA ends were isolated and subjected to proteomics analysis.

OTHER RELATED OMICS DATASETS IN: GSE126415

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Embryo, Early Embryonic Cell

SUBMITTER: Andreas Schmidt

LAB HEAD: Axel Imhof

PROVIDER: PXD012990 | Pride | 2019-10-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20151009_TT_loco_16_SWATH_oeb14.wiff	Wiff
	20151009_TT_loco_16_SWATH_oeb14.wiff.scan	Wiff
	20151009_TT_loco_17_SWATH_beb15.wiff	Wiff
	20151009_TT_loco_17_SWATH_beb15.wiff.scan	Wiff
	20151009_TT_loco_18_SWATH_beb13.wiff	Wiff

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Publications

A Drosophila cell-free system that senses DNA breaks and triggers phosphorylation signalling.

Harpprecht Lisa L Baldi Sandro S Schauer Tamas T Schmidt Andreas A Bange Tanja T Robles Maria S MS Kremmer Elisabeth E Imhof Axel A Becker Peter B PB

Nucleic acids research 20190801 14

Preblastoderm Drosophila embryo development is characterized by fast cycles of nuclear divisions. Extracts from these embryos can be used to reconstitute complex chromatin with high efficiency. We now discovered that this chromatin assembly system contains activities that recognize unprotected DNA ends and signal DNA damage through phosphorylation. DNA ends are initially bound by Ku and MRN complexes. Within minutes, the phosphorylation of H2A.V (homologous to γH2A.X) initiates from DNA breaks a ...[more]

PMID: 31147711

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