Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Monocyte
DISEASE(S): Acute Leukemia
SUBMITTER: Andrew Griswold
LAB HEAD: Daniel A. Bachovchin
PROVIDER: PXD013019 | Pride | 2019-04-23
REPOSITORIES: Pride
Griswold Andrew R AR Cifani Paolo P Rao Sahana D SD Axelrod Abram J AJ Miele Matthew M MM Hendrickson Ronald C RC Kentsis Alex A Bachovchin Daniel A DA
Cell chemical biology 20190418 6
The dipeptidyl peptidases (DPPs) regulate hormones, cytokines, and neuropeptides by cleaving dipeptides after proline from their amino termini. Due to technical challenges, many DPP substrates remain unknown. Here, we introduce a simple method, termed CHOPS (chemical enrichment of protease substrates), for the discovery of protease substrates. CHOPS exploits a 2-pyridinecarboxaldehyde (2PCA)-biotin probe, which selectively biotinylates protein N-termini except those with proline in the second po ...[more]