Proteomics

Dataset Information

0

Eukaryotic Ribosome GCN2 interaction Hydrogen/Deuterium Exchange Mass Spectrometry

ABSTRACT: General control nonderepressible 2 (GCN2) phosphorylates eIF2α, regulating translation in response to nutritional stress. Here, we show that mammalian ribosomes are potent GCN2 activators. Hydrogen/deuterium exchange–mass spectrometry (HDX-MS) showed GCN2 interacting with domain II of the uL10 P-stalk protein. The P-stalk is a uL10/P12/P22 pentameric complex that is part of the ribosomal GTPase-associated center. Recombinant human P-stalk greatly stimulates GCN2. Both domain II of uL10 and the C-terminal tails of P1 and P2 are necessary for maximal GCN2 activation. On actively translating ribosomes, the C-terminal tails of P1 and P2 are sequestered by elongation factors, suggesting P-stalk availability could link translational stress to GCN2 activation.

INSTRUMENT(S): Synapt MS

ORGANISM(S): Oryctolagus Cuniculus (rabbit)

TISSUE(S): Blood Cell, Blood

SUBMITTER: Glenn Masson  

LAB HEAD: Roger L. Williams

PROVIDER: PXD013051 | Pride | 2019-05-24

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
170717_Ribo_32oC_5m_1.raw.zip Raw
170717_Ribo_32oC_5m_2.raw.zip Raw
170717_Ribo_32oC_5m_3.raw.zip Raw
170717_Ribo_GCN2_32oC_5m_1.raw.zip Raw
170717_Ribo_GCN2_32oC_5m_2.raw.zip Raw
Items per page:
1 - 5 of 27
altmetric image

Publications

Activation of GCN2 by the ribosomal P-stalk.

Inglis Alison J AJ   Masson Glenn R GR   Shao Sichen S   Perisic Olga O   McLaughlin Stephen H SH   Hegde Ramanujan S RS   Williams Roger L RL  

Proceedings of the National Academy of Sciences of the United States of America 20190225 11

Cells dynamically adjust their protein translation profile to maintain homeostasis in changing environments. During nutrient stress, the kinase general control nonderepressible 2 (GCN2) phosphorylates translation initiation factor eIF2α, initiating the integrated stress response (ISR). To examine the mechanism of GCN2 activation, we have reconstituted this process in vitro, using purified components. We find that recombinant human GCN2 is potently stimulated by ribosomes and, to a lesser extent,  ...[more]

Similar Datasets

Genomics Developmentally regulated isoform of HNF4α reprograms the adult liver
2023-05-10 | GSE231538 | GEO
Transcriptomics Developmentally regulated isoform of HNF4α reprograms the adult liver
2018-08-31 | GSE117972 | GEO
Proteomics The HisRS-like domain of GCN2 is a pseudoenzyme that can bind uncharged tRNA - HX-MS Data
2024-02-01 | PXD049090 | Pride
Proteomics Conformational plasticity of RAS Q61 family of neoepitopes results in distinct features for targeted recognition.
2023-11-30 | PXD044838 | Pride
Proteomics,Multiomics ib101206 - proteome data analysis
2018-03-19 | PXD003287 | Pride
Proteomics Characterization of human sperm protamine proteoforms through a combination of top-down and bottom-up mass spectrometry approaches
2019-11-11 | PXD014618 | Pride
Proteomics Detailing the Structural and Dynamic Interactions Between WDR44 and Rab11
2023-03-11 | PXD035741 | Pride
Proteomics,Multiomics Structure dynamics of ApoA-I amyloidogenic variants in small HDL increase their ability to mediate cholesterol efflux
2020-11-20 | PXD021266 | Pride
Transcriptomics RNAseq for subpopulations of adipose tissue stromal cells from mouse inguinal adipose tissue
2021-12-07 | E-MTAB-9827 | biostudies-arrayexpress
Proteomics Resolving chaperone-assisted protein folding on the ribosome at the peptide level
2024-07-12 | PXD036945 | Pride