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Systems analyses reveal the remarkable resilience of Escherichia coli physiology during accumulation and export of the non-native organic acid citramalate

ABSTRACT: Proteomics part of multi-omic analyses of the citramalate fermentation were undertaken to uncover the reasons for remarkable citramalate productivity in an engineered strain of E. coli.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Escherichia Coli

SUBMITTER: David-Paul Minde

LAB HEAD: Prof. Kathryn S Lilley

PROVIDER: PXD013088 | Pride | 2019-06-05

REPOSITORIES: Pride

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Publications

Systems Analyses Reveal the Resilience of Escherichia coli Physiology during Accumulation and Export of the Nonnative Organic Acid Citramalate.

Webb Joseph J Springthorpe Vicki V Rossoni Luca L Minde David-Paul DP Langer Swen S Walker Heather H Alstrom-Moore Amias A Larson Tony T Lilley Kathryn K Eastham Graham G Stephens Gill G Thomas Gavin H GH Kelly David J DJ Green Jeffrey J

mSystems 20190611 4

Productivity of bacterial cell factories is frequently compromised by stresses imposed by recombinant protein synthesis and carbon-to-product conversion, but little is known about these bioprocesses at a systems level. Production of the unnatural metabolite citramalate in <i>Escherichia coli</i> requires the expression of a single gene coding for citramalate synthase. Multiomic analyses of a fermentation producing 25 g liter<sup>-1</sup> citramalate were undertaken to uncover the reasons for it ...[more]

PMID: 31186337

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