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Analysis of FucT and XylT1B mutants in C. reinhardtii

ABSTRACT: Secreted proteins found in the supernatant of different mutants were analyzed by mass spectrometry employing In source-CID. After analysis of intact N-glycopeptides on MS1 level, N-glycan compositions found were compared between two strains, consequently unraveling the molecular function of enzymes knocked down. Hereby, the role of a putative fucosyltransferase and the identity of a second core- xylosyltransferase could be confirmed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Chlamydomonas Reinhardtii

TISSUE(S): Culture Supernatant, Photosynthetic Cell

SUBMITTER: Anne Oltmanns

LAB HEAD: Michael Hippler

PROVIDER: PXD012107 | Pride | 2019-11-19

REPOSITORIES: Pride

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			Action	DRS
	AO-PLL_3x_xxf_ISCID_01022018_2p2k.mzML	Mzml
	AO-PLL_3x_xxf_ISCID_22012018_2p8k.mzML	Mzml
	AO-PLL_4x_xxfm_ISCID_01022018_2p2k.mzML	Mzml
	AO-PLL_4x_xxfm_ISCID_22012018_2p8k.mzML	Mzml
	AO_23022018_3x_XXF_I_4mg_2p3kV.mzML	Mzml

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