Dataset Information

Quantitative proteomic map of the trypanosomatid Strigomonas culicis: the biological contribution of its endosymbiotic bacterium

ABSTRACT: Strigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, that is highly integrated into the protozoa metabolism: it furnishes essential compounds and divides in synchrony with the nuclear host. The protozoa, conversely, can be rid of the endosymbiont, producing a cured cell line, which presents a diminished ability to colonize the insect host. This obligatory association can represent an intermediate step of the evolution towards the formation of a organelle, therefore representing an interesting model to understand the symbiogenesis theory. Here, we used shotgun proteomics to compare the S. culicis endosymbiont-containing and aposymbiotic strains, revealing a total of 11,305 peptides, and up to 2,213 proteins (2,029 and 1,452 for wild and aposymbiotic, respectively). Gene ontology associated to comparative analysis between both strains revealed that the biological processes most affected by the elimination of the symbiont were the amino acid metabolism, as well as protein synthesis and folding. This large-scale comparison of the protein expression in S. culicis marks a step forward in the comprehension of the role of endosymbiotic bacterium in monoxenic trypanosomatid biology, particularly because these organisms have a polycistronic open reading frame organization and post-transcriptional gene regulation.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Strigomonas Culicis

TISSUE(S): Cell Suspension Culture, Anatomical Entity

DISEASE(S): Disease Free

SUBMITTER: Richard Hemmi Valente

LAB HEAD: Richard Hemmi Valente

PROVIDER: PXD013136 | Pride | 2019-11-08

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Apo_24h_Rep02_Run_01.RAW	Raw
	Apo_24h_Rep02_Run_01.sqt	Other
	Apo_24h_Rep02_Run_02.RAW	Raw
	Apo_24h_Rep02_Run_02.sqt	Other
	Apo_24h_Rep02_Run_03.RAW	Raw

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Publications

Quantitative Proteomic Map of the Trypanosomatid Strigomonas culicis: The Biological Contribution of its Endosymbiotic Bacterium.

Brunoro Giselle V F GVF Menna-Barreto Rubem F S RFS Garcia-Gomes Aline S AS Boucinha Carolina C Lima Diogo B DB Carvalho Paulo C PC Teixeira-Ferreira André A Trugilho Monique R O MRO Perales Jonas J Schwämmle Veit V Catanho Marcos M de Vasconcelos Ana Tereza R ATR Motta Maria Cristina M MCM d'Avila-Levy Claudia M CM Valente Richard H RH

Protist 20191101 6

Strigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, which is highly integrated into the protist metabolism: it furnishes essential compounds and divides in synchrony with the eukaryotic nucleus. The protist, conversely, can be cured of the endosymbiont, producing an aposymbiotic cell line, which presents a diminished ability to colonize the insect host. This obligatory association can represent an intermed ...[more]

PMID: 31760169

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