Proteomics

Dataset Information

0

A dynamic and combinatorial histone code drives malaria parasite asexual and sexual development


ABSTRACT: Histone post-translational modifications (PTMs) are frequently co-occurring on the same chromatin domains or even the same molecule. It is now established that these ‘histone codes’ are the result of cross-talk between enzymes that catalyse multiple PTMs with univocal readout as compared to these PTMs in isolation. Here, we performed a comprehensive identification and quantification of histone codes of the malaria parasite, Plasmodium falciparum. We used advanced quantitative middle-down proteomics to identify combinations of PTMs in both the proliferative, asexual stages and transmissible, sexual gametocyte stages of P. falciparum. We provide an updated, high-resolution compendium of 72 PTMs on H3 and H3.3, of which 30 newly identified. Several co-existing PTMs with unique stage distinction was identified, indicating that many of these combinatorial PTMs are associated to specific stages of the parasite life cycle. We focused on the code H3R17me2K18acK23ac for its unique presence in mature gametocytes; chromatin proteomics identified a gametocyte-specific SAGA-like effector complex including the transcription factor AP2-G2 which we associated to this specific histone code, as involved in regulating gene expression in mature gametocytes. Ultimately, this study unveils previously undiscovered histone PTMs and their functional relationship with co-existing partners. These results highlight that investigating chromatin regulation in the parasite using single histone PTM assays might overlook higher order gene regulation for distinct proliferation and differentiation processes.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Falciparum V1

TISSUE(S): Blood Cell, Cell Suspension Culture, Monocyte, Cell Culture

SUBMITTER: Simone Sidoli  

LAB HEAD: Simone Sidoli

PROVIDER: PXD030181 | Pride | 2022-05-19

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
20190616_MC_HVG_01.raw Raw
20190616_MC_HVG_02.raw Raw
20190616_MC_HVG_03.raw Raw
20190616_MC_HVG_04.raw Raw
20190616_MC_HVG_05.raw Raw
Items per page:
1 - 5 of 54
altmetric image

Publications

A Dynamic and Combinatorial Histone Code Drives Malaria Parasite Asexual and Sexual Development.

von Grüning Hilde H   Coradin Mariel M   Mendoza Mariel R MR   Reader Janette J   Sidoli Simone S   Garcia Benjamin A BA   Birkholtz Lyn-Marié LM  

Molecular & cellular proteomics : MCP 20220117 3


Histone posttranslational modifications (PTMs) frequently co-occur on the same chromatin domains or even in the same molecule. It is now established that these "histone codes" are the result of cross talk between enzymes that catalyze multiple PTMs with univocal readout as compared with these PTMs in isolation. Here, we performed a comprehensive identification and quantification of histone codes of the malaria parasite, Plasmodium falciparum. We used advanced quantitative middle-down proteomics  ...[more]

Similar Datasets

2022-09-14 | PXD031817 | Pride
2015-11-24 | E-GEOD-75295 | biostudies-arrayexpress
2016-06-16 | PXD002560 | Pride
2014-10-16 | E-GEOD-62364 | biostudies-arrayexpress
2022-08-12 | PXD033544 | Pride
2010-10-08 | PXD009946 | Pride
2024-07-11 | PXD050362 | Pride
2023-02-13 | PXD031448 | Pride
2022-02-07 | PXD018295 | Pride
2020-11-06 | PXD013535 | Pride