Dataset Information

Ubiquitinylation in cancer - LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function

ABSTRACT: Samples were subjected to sonication using Bioruptor (program mode – 30 sec on, 30 sec off, 10 cycles = 10 min). Samples were sonicated till a clear solution was obtained. After a short spin (5,000 rpm for 10 sec), the samples were heated for 10 minutes at 95°C at 300 rpm in a PCR 96 heating block. Samples were allowed to cool down and spun at 5,000 rpm for 10 sec. 1μl of chloroacetamide was added to the eluate and incubated at 37°C for 30 minutes at 500 rpm. Later the samples were spun down at 5,000 rpm for 10 sec. Proteins were subjected to endoproteinase LysC (1:100 (w/w), Wako) digestion at 37°C for 4 hrs. Later, the proteins were subjected to trypsin digestion (0.5 μg/ μl; 1:50; w/w) at 37 °C overnight. Digestion was stopped by adding 50 μl of 5% TFA (Applied Biosystems) (v/v) that lowered the pH of the solution to below pH 2.0. Subsequently, peptides were cleaned up using the Phoenix 96x (https://preomics.com) kit following the manufacture’s instructions. After drying the peptides in a SpeedVac, samples were stored at -80°C.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Alexander Schmidt

LAB HEAD: Alexander Schmidt

PROVIDER: PXD013159 | Pride | 2020-01-13

REPOSITORIES: Pride

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LATS1 but not LATS2 represses autophagy by a kinase-independent scaffold function.

Tang Fengyuan F Gao Ruize R Jeevan-Raj Beena B Wyss Christof B CB Kalathur Ravi K R RKR Piscuoglio Salvatore S Ng Charlotte K Y CKY Hindupur Sravanth K SK Nuciforo Sandro S Dazert Eva E Bock Thomas T Song Shuang S Buechel David D Morini Marco F MF Hergovich Alexander A Matthias Patrick P Lim Dae-Sik DS Terracciano Luigi M LM Heim Markus H MH Hall Michael N MN Christofori Gerhard G

Nature communications 20191217 1

Autophagy perturbation represents an emerging therapeutic strategy in cancer. Although LATS1 and LATS2 kinases, core components of the mammalian Hippo pathway, have been shown to exert tumor suppressive activities, here we report a pro-survival role of LATS1 but not LATS2 in hepatocellular carcinoma (HCC) cells. Specifically, LATS1 restricts lethal autophagy in HCC cells induced by sorafenib, the standard of care for advanced HCC patients. Notably, autophagy regulation by LATS1 is independent of ...[more]

PMID: 31848340

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			Action	DRS
	001_HSK-Ub1_180703225807_C18.raw	Raw
	001_HSK-Ub1_C18.raw	Raw
	002_HSK-Ub1_180704192956_C18.raw	Raw
	002_HSK-Ub1_C18.raw	Raw
	003_HSK-Ub1_C18.raw	Raw