Dataset Information

Proteomics identification and validation of desmocollin-1 and catechol-O-methyltransferase as proteins associated with breast cancer cell migration and metastasis

ABSTRACT: Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, we selected and characterized a more migrating subpopulation of MDA-MB-231 breast cancer cells. We applied quantitative mass spectrometry with SILAC labeling to analyze their surfaceome and compared it with that of paternal MDA-MB-231 cells. Among 818 identified proteins (FDR < 0.01), 124 differentially abundant cell surface proteins with at least one transmembrane domain were found. Of these, (i) catechol-O-methyltransferase (COMT) was successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as tumor grade by targeted data extraction from the SWATH-MS data set of 96 breast cancer tissues, and (ii) desmocollin-1 (DSC1) was verified as a protein connected with lymph node status of luminal A breast cancer, tumor grade and Her-2 receptor by immunohistochemistry in the same set of patients. Kaplan-Meier analysis associated COMT and DSC1 with decreased overall survival in breast cancer subsets. Our findings indicate importance of both proteins for breast cancer development and metastasis

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Breast Cancer

SUBMITTER: Pavel Bouchal

LAB HEAD: Pavel Bouchal

PROVIDER: PXD013162 | Pride | 2019-10-23

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Andromeda_Peak_Lists.zip	Other
	Bouchal_130104_SILAC-1_KE_1x_02.raw	Raw
	Bouchal_130104_SILAC-2_KE_1x_02.raw	Raw
	Bouchal_130104_SILAC-3_KE_1x_02.raw	Raw
	Bouchal_130104_SILAC-4_KE_1x_02.raw	Raw

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Publications

Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis.

Faktor Jakub J Knopfova Lucia L Lapcik Petr P Janacova Lucia L Paralova Vendula V Bouchalova Pavla P Muller Petr P Benes Petr P Bouchal Pavel P

Proteomics 20191028 21-22

Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified prot ...[more]

PMID: 31617665

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