Dataset Information

Differential proteomics of the synaptosomal fraction from rat dentate gyrus identified the changes associated with aging-dependent cognitive decline

ABSTRACT: The study contained three groups of Sprague-Dawley male rats – two groups of aging animals (22-24 months) separated based on performance in spatial reference memory task; and the third, control group of young animals () not separated by behavioural testing. The synaptosome – enriched membrane fraction of the dentate gyrus was isolated from nine randomly chosen individuals per group and analysed by tandem mass tag (TMT) labelling and mass spectrometry (MS)-based quantitative proteomics. The proteomic experiment was performed in three TMT10plex sets, with each set containing samples from three biological replicates per group and one TMT 126 reference channel reserved to directly compare the protein intensities across the entire study. The peptides were analysed in two parallel MS runs representing two technical replicates per biological sample. The total number of 6513 protein entries was identified of which 471 showed a significantly changed level (p<0.05) in any of the three animal groups. The comparison between aged and young animals revealed 293 significantly changed proteins (p<0.05) in aged good performers vs young rats, and 310 significant proteins (p < 0.05) in aged bad performers vs young ones. The proteomic differences between aged good vs bad performers were represented by 78 significantly changed proteins (p < 0.05).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Dentate Gyrus, Dentate Gyrus Of Hippocampal Formation

SUBMITTER: Gert Lubec

LAB HEAD: Gert Lubec

PROVIDER: PXD013172 | Pride | 2019-08-19

REPOSITORIES: Pride

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	NP25-Or11F1-BC1-P1491-5.raw	Raw
	NP25-Or11F1-BC1-P1491-6.raw	Raw
	NP25-Or11F2-BC2-P1492-5.raw	Raw
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Publications

Dentate Gyrus Peroxiredoxin 6 Levels Discriminate Aged Unimpaired From Impaired Rats in a Spatial Memory Task.

Lubec Jana J Smidak Roman R Malikovic Jovana J Feyissa Daniel Daba DD Korz Volker V Höger Harald H Lubec Gert G

Frontiers in aging neuroscience 20190731

Similar to humans, the normal aged rat population is not homogeneous in terms of cognitive function. Two distinct subpopulations of aged Sprague-Dawley rats can be identified on the basis of spatial memory performance in the hole-board paradigm. It was the aim of the study to reveal protein changes relevant to aging and spatial memory performance. Aged impaired (AI) and unimpaired (AU) male rats, 22-24 months old were selected from a large cohort of 160 animals; young animals served as control. ...[more]

PMID: 31417400

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