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MSCs on soft/stiff substrates treated with ROCK or myosin II inhibitors

ABSTRACT: Tissues are maintained by homeostatic feedback mechanisms in which cells can respond to, but also modify, the chemical and mechanical properties of the surrounding extracellular matrix (ECM). Mechano-sensitive mesenchymal stem cells (MSCs) resident in the marrow niche experience a diverse mechanical environment, but ageing can affect the composition and quality of bone and marrow tissues. Here we quantified the compounded effects of substrate stiffness and replication-induced senescence on MSC morphology and their ability to modify their environments through secretion of ECM proteins. The ECM proteome was found to be sensitive to substrate stiffness, but pharmacological inhibition of cellular contractility perturbed this response, decreasing levels of tenascin-C, fibulins and fibronectin. A corresponding change in the ECM of senescent cells, concomitant with a loss of mechano-responsive morphological features, suggested a decoupling of mechanotransduction pathways. Intracellular proteomic and transcriptomic analyses confirmed a decrease in all components of the cytoskeletal protein homeostasis machinery in senescent MSCs. These results demonstrate a senescence-mediated perturbation to cytoskeletal homeostasis, pathways of mechanotransduction and the secretion of ECM proteins considered necessary for tissue maintenance.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Bone Marrow

SUBMITTER: Venkatesh Mallikarjun

LAB HEAD: Joe Swift

PROVIDER: PXD013239 | Pride | 2022-10-12

REPOSITORIES: Pride

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			Action	DRS
	20151029_JS_7.raw	Raw
	20151029_JS_8.raw	Raw
	20151029_JS_9.raw	Raw
	20151218_JS_1.raw	Raw
	20151218_JS_16.raw	Raw

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